| Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads. | |
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MedLine Citation:
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PMID: 15626629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bondforces between biotinylated surfaces and streptavidin or avidin coated beads are investigated by a magnetic field based manipulation system for magnetic microbeads. The magnetic field is generated by currents through a set of conducting lines, and its gradient exerts a force onto the magnetic beads. The force can be increased until the bond between the bead and the surface breaks. Consistent with other groups we found two conformations for both investigated bonds. The measured bondforces for the two conformations are for Streptavidin-Biotin: 55.9 and 244.7 fN and for Avidin-Biotin: 15.9 and 58.4 fN. These very low bondforces (10-100 times smaller than earlier measurements) match to the extremely low loading rate of about 1 fN/s. This new technique thus allows to investigate biomolecular bonds by extremely low forces. |
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Authors:
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Michael Panhorst; Paul-Bertram Kamp; Günter Reiss; Hubert Brückl |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 20 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2004-12-31 Completed Date: 2005-06-09 Revised Date: 2009-07-14 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 1685-9 Citation Subset: IM |
Affiliation:
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University of Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany. Michael.Panhorst@Physik.Uni-Bielefeld.DE |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Avidin
/
analysis,
chemistry*,
ultrastructure Binding Sites Biotin / analysis, chemistry* Magnetics* Micromanipulation / instrumentation, methods* Microspheres Physical Stimulation / instrumentation, methods Protein Binding Reproducibility of Results Sensitivity and Specificity Streptavidin / analysis, chemistry*, ultrastructure Stress, Mechanical |
| Chemical | |
Reg. No./Substance:
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1405-69-2/Avidin; 58-85-5/Biotin; 9013-20-1/Streptavidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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