Document Detail


Sensitive assay systems for detection of hemoglobin with 2,7-diaminofluorene: histochemistry and colorimetry for erythrodifferentiation.
MedLine Citation:
PMID:  2416239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sensitive and rapid assays, colorimetry and histochemistry, for hemoglobin in erythroid cells are established. The assays are based on pseudoperoxidase activity of hemoglobin using 2,7-diaminofluorene as a hydrogen donor for the peroxidase, instead of benzidine which is widely benzidine which is widely used for the detection of small amounts of hemoglobin but which is a potent carcinogen and has been banned from laboratory use. In the presence of hydrogen peroxide, hemoglobin catalyzes the formation of a blue compound (fluorene blue), which has a broad absorption band between 500 and 690 nm with a peak at 610 nm, from 2,7-diaminofluorene. The reagent is safe to use in the laboratory. The methods could be applied to the detection of hemoglobin in Friend erythroleukemia cells induced to cell differentiation along the erythroid pathway by dimethyl sulfoxide.
Authors:
S Kaiho; K Mizuno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  149     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1986-01-14     Completed Date:  1986-01-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzidines
Cell Differentiation
Cell Line
Colorimetry
Dimethyl Sulfoxide
Fluorenes*
Friend murine leukemia virus
Hemoglobins / analysis*
Histocytochemistry
Leukemia, Erythroblastic, Acute / blood*
Leukemia, Experimental
Mice
Staining and Labeling
Chemical
Reg. No./Substance:
0/Benzidines; 0/Fluorenes; 0/Hemoglobins; 525-64-4/2,7-fluorenediamine; 67-68-5/Dimethyl Sulfoxide; 92-87-5/benzidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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