Document Detail


Sensitive assay system for bile acids and steroids having hydroxyl groups utilizing high-performance liquid chromatography with peroxyoxalate chemiluminescence detection.
MedLine Citation:
PMID:  2283379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
3 alpha- or 3 beta-hydroxysteroids, such as bile acids (free and glycine and taurine conjugates), 3 beta-hydoxy-5-cholenic acid, pregnanediol, 5-pregnene-3 beta, 20 beta-diol and 5-pregnene-3-beta,20 alpha-diol, were converted to 3-oxosteroids by enzymatic reaction using immobilized hydroxysteroid dehydrogenase, derivatized with dansylhydrazine to the corresponding dansyl hydrazones and purified by gel permeation chromatography. The dansyl hydrazones were chromatographed on a C18 column with a tetrahydrofuran-containing eluent and detected at the level of a few femtomoles by a peroxyoxalate chemiluminescence post-column reaction using bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] oxalate as a chemilumigenic reagent. The dansyl hydrazones of chenodeoxycholic acid and deoxycholic acid (free and glycine and taurine conjugates) in particular, which coeluted under the chromatographic conditions above, were separated using an eluent including acetonitrile and 2,6-di-O-methyl-beta-cyclodextrin and detected in the same way.
Authors:
S Higashidate; K Hibi; M Senda; S Kanda; K Imai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  515     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1991-03-15     Completed Date:  1991-03-15     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  577-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / analysis*
Chromatography, High Pressure Liquid / methods*
Dansyl Compounds / diagnostic use
Hydrazines / diagnostic use
Hydroxysteroids / analysis*
Luminescent Measurements*
Oxalates / diagnostic use*
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Dansyl Compounds; 0/Hydrazines; 0/Hydroxysteroids; 0/Oxalates; 33008-06-9/dansyl hydrazine; 5796-84-9/peroxyoxalate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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