Document Detail


Sensitive antiproliferative neutralization assay for the detection of neutralizing IFN-alpha and IFN-beta antibodies.
MedLine Citation:
PMID:  8176238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antibodies to interferon (IFN) may compromise IFN treatment in some patients. In tumor therapy, a critical function of type I IFNs is their antiproliferative effect. For the quantification of neutralizing IFN antibodies we have developed an antiproliferative neutralization assay (APA) based on the reduction of IFN-mediated growth inhibition of Daudi cells by IFN-alpha and IFN-beta antibodies. Proliferation was quantified by [3H]thymidine incorporation, and the neutralizing potency of IFN antibody-positive sera was expressed as the neutralizing titer inhibiting 50% of the antiproliferative activity of 10 IU/ml of IFN (NT50). The APA is easy to perform, reproducible, and more sensitive than a well-established antiviral neutralization assay (AVA). All 30 sera with recombinant IFN-alpha 2a-binding antibodies proved to be neutralizing antibody-positive in the APA whereas seven were scored antibody-negative or uninterpretable in the AVA. The APA is recommended as a second or third line assay for the estimation of the neutralizing potency of spontaneous or treatment-induced IFN-alpha and IFN-beta-specific antibodies.
Authors:
O Prümmer; U Streichan; H Heimpel; F Porzsolt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  171     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-08     Completed Date:  1994-06-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  45-53     Citation Subset:  IM    
Affiliation:
Department of Medicine III, University of Ulm, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / blood*,  immunology
Antibody Specificity
Cattle
Cell Division / drug effects
Humans
Interferon Type I, Recombinant / antagonists & inhibitors*,  immunology,  pharmacology
Interferon-beta / antagonists & inhibitors*,  immunology,  pharmacology
Kinetics
Neutralization Tests / methods*
Pilot Projects
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies; 0/Interferon Type I, Recombinant; 77238-31-4/Interferon-beta

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