| Sensitive Detection Assays for Influenza RNA Do Not Reveal Viremia in US Blood Donors. | |
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MedLine Citation:
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PMID: 22293429 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background. There have been anecdotal reports of influenza viremia since the 1960s. We present an assessment of the prevalence of seasonal and 2009 H1N1 influenza viremia (via RNA testing) in blood donor populations using multiple sensitive detection assays.Methods. Several influenza RNA amplification assays, including transcription-mediated amplification (TMA) and 2 reverse-transcription polymerase chain reaction (RT-PCR) assays, were evaluated and used to test donor samples. Retrospective samples from 478 subjects drawn at sites with high influenza activity were tested. Prospective samples were collected from 1004 blood donors who called their donation center within 3 days of donation complaining of influenza-like illness (ILI). The plasma collected on the day of donation for these subjects was tested.Results. Of the repository samples, 2 of 478 plasma samples were initially reactive but not repeat reactive by influenza TMA. Of blood donors reporting ILI symptoms postdonation, 1 of 1004 samples was TMA initially reactive but not repeat reactive; all samples were nonreactive by RT-PCR testing.Conclusions. Targeting blood donor populations most likely to have influenza infection, we failed to detect influenza RNA in 1482 donor samples, with most tested by 3 different RNA assays. Seasonal influenza does not appear to pose a significant contamination threat to the blood supply. |
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Authors:
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Susan L Stramer; Cynthia Collins; Thomas Nugent; Xue Wang; Meghan Fuschino; John W Heitman; Jacqueline Law; David E Krysztof; Nancy Kiely; Deborah Todd; Nicolaas M J Vermeulen; Karen Harrington; Hany Kamel; David J Kelvin; Michael P Busch; Kirsten St George; Indira K Hewlett; Jeffrey M Linnen; Philip J Norris; |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-31 |
Journal Detail:
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Title: The Journal of infectious diseases Volume: - ISSN: 1537-6613 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-2-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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American Red Cross Scientific Support Office, Gaithersburg, Maryland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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