Document Detail

Sensitive Cylindrical SERS Substrate Array for Rapid Microanalysis of Nucleobases.
MedLine Citation:
PMID:  23140099     Owner:  NLM     Status:  Publisher    
In this work, a cylindrical-substrate array for surface-enhanced Raman scattering (SERS) measurements was developed to enable analysis of nucleobases in a few microliters of liquid. To eliminate uncertainties associated with SERS detection of aqueous samples, a new type of cylindrical SERS substrate was designed to confine the aqueous sample at the tip of the SERS probe. Polymethyl methacrylate (PMMA) optical fibers in a series of different diameters were used as the basic substrate. A solution of polyvinylidene fluoride/ dimethylformamide (PVDF/DMF) was used to coat the tip of each fiber to increase the surface roughness and facilitate adsorption of silver nanoparticles (AgNPs) for enhancing Raman signals. A chemical reduction method was used to form AgNPs in and on the PVDF coating layer. The reagents and reaction conditions were systematically examined with the aim of estimating the optimum parameters. Unlike the spreading of aqueous sample on most SERS substrates, particularly flat ones, the new SERS substrates showed enough hydrophobicity to restrict aqueous sample to the tip area, thus enabling quantitative analysis. The required volume of sample could be as low as 1 μL with no need for a drying step in the procedure. By aligning the cylindrical SERS substrates into a solid holder, an array of cylindrical-substrates was produced for mass analysis of aqueous samples. This new substrate improves both reproducibility and sensitivity for detection in aqueous samples. The enhancement factor approaches 7 orders in magnitude with a relative standard error close to 8%. Using the optimized conditions, nucleobases of adenine, cytosine, thymine and uracil could be detected with limits approaching few hundreds nanomolar in only a few microliters of solution.
Panneerselvam Rajapandiyan; Jyisy Yang
Related Documents :
23527539 - Properties and water sorption characteristics of spaghetti prepared using various dies.
18824689 - Thresholds of hypoxia for marine biodiversity.
23246339 - Characterization of a new tif(4) and β-cyclodextrin inclusion complex and its in vitro...
24632369 - Forensic source differentiation of petrogenic, pyrogenic, and biogenic hydrocarbons in ...
15993139 - Use of composite data sets for source-tracking enterococci in the water column and shor...
23712559 - Understanding crystallization features of p(vdf-trfe) copolymers under confinement to o...
22599489 - Eradicating legionella from hospital water-reply.
23416619 - Chemical and spectroscopic characterization of water extractable organic matter during ...
22325429 - Ozone pollution affects flower numbers and timing in a simulated bap priority calcareou...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-9
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Transcriptomic and phylogenetic analysis of Culex pipiens quinquefasciatus for three detoxification ...
Next Document:  Social, psychological and economic challenges faced by transgender individuals and their significant...