Document Detail


Sensitisation of TRPV1 in rat sensory neurones by activation of SNSRs.
MedLine Citation:
PMID:  17601667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The novel sensory neurone specific receptor (SNSR) family of G-protein coupled receptors are activated by non-opiate fragments of opioid precursor peptides. SNSRs are expressed in nociceptors, and SNSR agonists have been found to cause sensitisation to painful stimuli in vivo. We explored the basis of sensitisation caused by SNSR agonists in sensory neurones by investigating the effect of the SNSR-selective agonist bovine adrenal medulla peptide 8-22 (BAM (8-22)) on gating of the heat and capsaicin-sensitive ion channel TRPV1. Using calcium imaging we found that BAM (8-22) caused sensitisation of the TRPV1 response in approximately 13% of DRG neurones. Sensitisation of TRPV1 in a similar proportion of neurones was observed using whole-cell patch clamping. The PKC-specific inhibitor Ro-31-8220 reduced but did not completely abolish sensitisation, while the protein kinase A inhibitor H-89 was without significant effect. No translocation of the PKC delta, epsilon and zeta isoforms to the cell membrane was observed in response to BAM (8-22). These observations implicate PKC in the sensitisation of TRPV1, but suggest that other pathways are also involved.
Authors:
Stuart Andrew Honan; Peter Anthony McNaughton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-02
Journal Detail:
Title:  Neuroscience letters     Volume:  422     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-24     Completed Date:  2007-09-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analogs & derivatives,  pharmacology
Animals
Calcium / metabolism
Capsaicin / pharmacology
Cattle
Enkephalin, Methionine / analogs & derivatives,  pharmacology
Ganglia, Spinal / cytology,  physiology
Hot Temperature
Immunohistochemistry
Indoles / pharmacology
Isoenzymes / antagonists & inhibitors,  metabolism
Isoquinolines / pharmacology
Membrane Potentials / physiology
Neurons, Afferent / drug effects,  physiology*
Nociceptors / drug effects,  physiology
Patch-Clamp Techniques
Protein Kinase C / antagonists & inhibitors,  metabolism
Protein Precursors / pharmacology
Rats
Signal Transduction / drug effects,  physiology
Sulfonamides / pharmacology
TRPV Cation Channels / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Indoles; 0/Isoenzymes; 0/Isoquinolines; 0/Protein Precursors; 0/Sulfonamides; 0/TRPV Cation Channels; 0/Trpv1 protein, rat; 125314-64-9/Ro 31-8220; 127243-85-0/H 89; 404-86-4/Capsaicin; 56-65-5/Adenosine Triphosphate; 58569-55-4/Enkephalin, Methionine; 7292-42-4/alpha,beta-methyleneadenosine 5'-triphosphate; 7440-70-2/Calcium; 76622-26-9/BAM 22P; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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