Document Detail


Sensitisation of Ehrlich ascitic tumour cells to methotrexate by inhibiting glutaminase.
MedLine Citation:
PMID:  16101144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Glutaminase activity is correlated with cancer proliferation and with growth rate in normal cells. Ehrlich ascites tumour cells (EATC) and their derivative 0.28AS-2 cells, which express antisense glutaminase mRNA, show differences in both morphology and tumorigenic capacity. MATERIALS AND METHODS: Cell viability was determined with the microtetrazolium cytotoxicity test assay. Immunofluorescence staining with annexin-V and propidium iodide was carried out to assess the number of apoptotic cells. RESULTS: 0.28AS-2 cells are less resistant to H2O2 than EATC, since half the concentration of H2O2 caused a similar effect on the cell population in 24 h. Methotrexate significantly inhibited the proliferation of both EATC and 0.28AS-2 cells at concentrations higher than 64 nM after 48 h of exposure. CONCLUSION: 0.28AS-2 cells are highly sensitised to methotrexate. These results provide insights into the possible role of glutaminase in cancer therapy by demonstrating that the expression of antisense mRNA for glutaminase decreases chemoresistance to some pro-apoptotic agents.
Authors:
Francisco J Alonso; Juan A Segura; Jorge Lora; Carolina Lobo; Beatriz Fernández-Molina; Javier Márquez; José M Matés
Related Documents :
22519634 - Cryopreservation of umbilical cord mesenchymal cells in xenofree conditions.
6634584 - Ascites formation in the chicken.
3609324 - Acidification of the interior of ehrlich ascites tumor cells by nigericin inhibits dna ...
20689764 - Effect of ovarian cancer ascites on cell migration and gene expression in an epithelial...
22827384 - Life or death decisions in the corpus luteum.
17890904 - Development of fetal testicular cells in androgen receptor deficient mice.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  25     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2005 Sep-Oct
Date Detail:
Created Date:  2005-08-16     Completed Date:  2005-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3315-20     Citation Subset:  IM    
Affiliation:
Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antimetabolites, Antineoplastic / pharmacology*
Apoptosis / drug effects
Carcinoma, Ehrlich Tumor / drug therapy*,  enzymology*,  pathology
Cell Growth Processes / drug effects
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Synergism
Glutaminase / antagonists & inhibitors*,  genetics
Hydrogen Peroxide / pharmacology*
Methotrexate / pharmacology*
RNA, Antisense / genetics
RNA, Messenger / genetics
Chemical
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/RNA, Antisense; 0/RNA, Messenger; 59-05-2/Methotrexate; 7722-84-1/Hydrogen Peroxide; EC 3.5.1.2/Glutaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Blockade of B7-H1 with sPD-1 improves immunity against murine hepatocarcinoma.
Next Document:  Proteasome inhibitors abolish cell death downstream of caspase activation during anti-microtubule dr...