| Sensing intracellular oxygen using near-infrared phosphorescent probes and live-cell fluorescence imaging. | |
| | |
MedLine Citation:
|
PMID: 17170232 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The development and application of a methodology for measurement of oxygen within single mammalian cells are presented, which employ novel macromolecular near infrared (NIR) oxygen probes based on new metalloporphyrin dyes. The probes, which display optimal spectral characteristics and sensitivity to oxygen, excellent photostability, and low cytotoxicity and phototoxicity, are loaded into cells by simple transfection procedures and subsequently analyzed by high-resolution fluorescence microscopy. The methodology is demonstrated by sensing intracellular oxygen in different mammalian cell lines, including A549, Jurkat, and HeLa, and monitoring rapid and transient changes in response to mitochondrial uncoupling by valinomycin and inhibition by antimycin A. Furthermore, the effect of ryanodine receptor-mediated Ca(2+) influx on cellular oxygen uptake is shown by substantial changes in the level of intracellular oxygen. The results demonstrate the ability of this technique to measure small, rapid, and transient changes in intracellular oxygen in response to different biological effectors. Moreover, this technique has wide ranging applicability in cell biology and is particularly useful in the study of low oxygen environments (cellular hypoxia), mitochondrial and cellular (dys)function, and for therapeutic areas, such as cardiovascular and neurological research, metabolic diseases, and cancer. |
| | |
Authors:
|
Tomás C O'Riordan; Kathleen Fitzgerald; Gelii V Ponomarev; John Mackrill; James Hynes; Cormac Taylor; Dmitri B Papkovsky |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-14 |
Journal Detail:
|
Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 292 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2007 Apr |
Date Detail:
|
Created Date: 2007-04-04 Completed Date: 2007-05-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: R1613-20 Citation Subset: IM |
Affiliation:
|
Luxcel Biosciences, BioInnovation Centre, University College Cork, Cork, Ireland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antimycin A
/
pharmacology Biosensing Techniques / methods* Cell Line, Tumor Fluorescence* Fluorescent Dyes / metabolism Hela Cells Humans Indoles / metabolism Ionophores / pharmacology Jurkat Cells Kinetics Luminescent Agents Luminescent Measurements* Lung Neoplasms / pathology Metalloporphyrins / metabolism Microscopy, Confocal Microscopy, Fluorescence Mitochondria / drug effects Oxygen / analysis* Spectroscopy, Near-Infrared* Valinomycin / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 0/Indoles; 0/Ionophores; 0/Luminescent Agents; 0/Metalloporphyrins; 2001-95-8/Valinomycin; 47165-04-8/DAPI; 642-15-9/Antimycin A; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of cytochrome oxidase redox state during umbilical cord occlusion in preterm fetal sheep.
Next Document: TNF-alpha neutralization ameliorates obstruction-induced renal fibrosis and dysfunction.