Document Detail


Sensing muscle ischemia: coincident detection of acid and ATP via interplay of two ion channels.
MedLine Citation:
PMID:  21092862     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ischemic pain--examples include the chest pain of a heart attack and the leg pain of a 30 s sprint--occurs when muscle gets too little oxygen for its metabolic need. Lactic acid cannot act alone to trigger ischemic pain because the pH change is so small. Here, we show that another compound released from ischemic muscle, adenosine tri-phosphate (ATP), works together with acid by increasing the pH sensitivity of acid-sensing ion channel number 3 (ASIC3), the molecule used by sensory neurons to detect lactic acidosis. Our data argue that ATP acts by binding to P2X receptors that form a molecular complex with ASICs; the receptor on sensory neurons appears to be P2X5, an electrically quiet ion channel. Coincident detection of acid and ATP should confer sensory selectivity for ischemia over other conditions of acidosis.
Authors:
William T Birdsong; Leonardo Fierro; Frank G Williams; Valeria Spelta; Ligia A Naves; Michelle Knowles; Josephine Marsh-Haffner; John P Adelman; Wolfhard Almers; Robert P Elde; Edwin W McCleskey
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuron     Volume:  68     ISSN:  1097-4199     ISO Abbreviation:  Neuron     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2011-01-06     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  United States    
Other Details:
Languages:  eng     Pagination:  739-49     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Vollum Institute, Oregon Health & Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97239-3098, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Sensing Ion Channels
Acidosis, Lactic / metabolism,  physiopathology
Adenosine Triphosphate / metabolism*,  physiology
Amino Acid Sequence
Animals
CHO Cells
COS Cells
Cercopithecus aethiops
Cricetinae
Cricetulus
HEK293 Cells
Humans
Hydrogen-Ion Concentration
Ischemia / metabolism*,  physiopathology
Molecular Sequence Data
Muscle, Skeletal / blood supply*,  innervation*,  metabolism
Rats
Rats, Sprague-Dawley
Receptors, Purinergic P2X5 / metabolism*,  physiology
Sensory Receptor Cells / metabolism*,  pathology,  physiology
Sodium Channels / metabolism*,  physiology
Grant Support
ID/Acronym/Agency:
HL64840/HL/NHLBI NIH HHS; NS37010/NS/NINDS NIH HHS; R01 HL064840-05/HL/NHLBI NIH HHS; R01 NS037010-08/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/ASIC3 protein, human; 0/Acid Sensing Ion Channels; 0/Receptors, Purinergic P2X5; 0/Sodium Channels; 56-65-5/Adenosine Triphosphate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bassoon and the synaptic ribbon organize Ca²+ channels and vesicles to add release sites and promot...
Next Document:  Visual deprivation suppresses L5 pyramidal neuron excitability by preventing the induction of intrin...