| Senescent phenotypes and telomere lengths of peripheral blood T-cells mobilized by acute exercise in humans. | |
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MedLine Citation:
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PMID: 20839490 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acute bouts of aerobic exercise are known to mobilize antigen-experienced CD8+ T-cells expressing the cell surface marker of senescence, KLRG1, into the blood. It is not known; however if this is due to a selective mobilization of terminally differentiated T-cells (i.e., KLRG1 +/CD28-/CD57+) or a population of effector memory T-cells (i.e., KLRG1+/CD28+/CD57-) that have not reached terminal differentiation. The aim of this study was to further characterize KLRG1 + T-cells mobilized by acute exercise by assessing the co-expression of KLRG1 with CD28 or CD57 and to determine telomere lengths in the CD4+ and CD8+ T-cell subsets. Nine moderately trained male subjects completed an exhaustive treadmill running protocol at 80%. Blood lymphocytes isolated before, immediately after and 1h after exercise were labelled with antibodies against KLRG1, CD28 or CD57, CD4 or CD8 and CD3 for 4-color flow cytometry analysis. Telomere lengths in CD3+, CD4+ and CD8+ T-cells were determined using Q-PCR. The relative proportion of KLRG1 + cells among the CD8+ T-cells increased by 40% immediately after exercise, returning to baseline 1h later. This was due to a mobilization of KLRG1+/CD28- (61% increase), KLRG1+/CD57+ (56% increase) and to a lesser extent, KLRG1+/CD57- cells (24% increase). Telomeres in CD8+ T-cells displayed an increased relative length immediately after exercise, whereas no change occurred for CD4+ or the overall CD3+ T-cells. In conclusion, the increased frequency of KLRG1 +/CD8+ T-cells in blood after acute exercise is predominantly due to a selective mobilization of terminally differentiated T-cells. The increased relative telomere length in CD8+ T-cells after exercise might indicate that KLRG1+ cells mobilized by exercise are under stress or aberrant signaling-induced senescence (STASIS). We postulate that a frequent mobilization of these cells by acute exercise might eventually allow naïve T-cells to occupy the "vacant" immune space and increase the naïve T-cell repertoire. |
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Authors:
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Richard J Simpson; Cormac Cosgrove; Meng M Chee; Brian K McFarlin; David B Bartlett; Guillaume Spielmann; Daniel P O'Connor; Hanspeter Pircher; Paul G Shiels |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Exercise immunology review Volume: 16 ISSN: 1077-5552 ISO Abbreviation: Exerc Immunol Rev Publication Date: 2010 |
Date Detail:
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Created Date: 2010-09-15 Completed Date: 2010-10-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9505535 Medline TA: Exerc Immunol Rev Country: Germany |
Other Details:
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Languages: eng Pagination: 40-55 Citation Subset: IM |
Affiliation:
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Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, Texas 77204, USA. rjsimpson@uh.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cell Aging / immunology* Cell Differentiation / immunology Cell Movement / immunology* Cell Separation Exercise* Flow Cytometry Humans Lectins, C-Type / biosynthesis, immunology Male Phenotype Reverse Transcriptase Polymerase Chain Reaction T-Lymphocyte Subsets / cytology*, immunology, metabolism T-Lymphocytes / cytology*, immunology, metabolism Telomere / immunology, metabolism* Trans-Activators / biosynthesis, immunology |
| Chemical | |
Reg. No./Substance:
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0/KLRG1 protein, human; 0/Lectins, C-Type; 0/Trans-Activators |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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