| Senescent keratinocytes die by autophagic programmed cell death. | |
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MedLine Citation:
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PMID: 19147823 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Normal cells reach senescence after a specific time and number of divisions, leading ultimately to cell death. Although escape from this fate may be a requisite step in neoplastic transformation, the mechanisms governing senescent cell death have not been well investigated. We show here, using normal human epidermal keratinocytes, that no apoptotic markers appear with senescence. In contrast, the expression of several proteins involved in the regulation of macroautophagy, notably Beclin-1 and Bcl-2, was found to change with senescence. The corpses occurring at the senescence growth plateau displayed a large central area delimited by the cytokeratin network that contained a huge quantity of autophagic vacuoles, the damaged nucleus, and most mitochondria. 3-methyladenine, an inhibitor of autophagosome formation, but not the caspase inhibitor zVAD, prevented senescent cell death. We conclude that senescent cells do not die by apoptosis, but as a result of high macroautophagic activity that targets the primary vital cell components. |
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Authors:
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Karo Gosselin; Emeric Deruy; Sébastien Martien; Chantal Vercamer; Fatima Bouali; Thibault Dujardin; Christian Slomianny; Ludivine Houel-Renault; Fazia Chelli; Yvan De Launoit; Corinne Abbadie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-15 |
Journal Detail:
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Title: The American journal of pathology Volume: 174 ISSN: 1525-2191 ISO Abbreviation: Am. J. Pathol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-23 Completed Date: 2009-02-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 423-35 Citation Subset: AIM; IM |
Affiliation:
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UMR8161, Institut de Biologie de Lille, Lille Cedex, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis Regulatory Proteins
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biosynthesis Autophagy / physiology* Blotting, Western Cell Aging / physiology Female Flow Cytometry Fluorescent Antibody Technique Gene Expression Humans In Situ Nick-End Labeling Keratinocytes / pathology*, physiology Membrane Proteins / biosynthesis Microscopy, Electron, Transmission Proto-Oncogene Proteins c-bcl-2 / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/BECN1 protein, human; 0/Membrane Proteins; 0/Proto-Oncogene Proteins c-bcl-2 |
| Comments/Corrections | |
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