| Senescent fibroblasts induce moderate stress in lung epithelial cells in vitro. | |
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MedLine Citation:
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PMID: 16600555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epithelial-mesenchymal interactions contribute to functionality and integrity of the lung epithelium, which might change during ageing and associated cellular ageing. Therefore, we studied the effect of senescent versus pre-senescent lung fibroblasts (WI-38) on mitogenic and stress-protective factors in lung epithelial cells (H358). By use of conditioned medium, we found a growth promoting impact of fibroblasts compared with control medium from epithelial cells associated with activation of ERK1/2, Akt, p70S6K, and EGF receptor. Although senescent fibroblasts mediated similar growth stimulation compared with pre-senescent cells, we observed less protection against spontaneous mitochondrial dysfunction in vitro, higher production of reactive oxygen species and activation of copper/zinc superoxide dismutase. Moreover, senescent cells induced activation of caspase-3/7 in epithelial cells, which was associated with down-regulation of the caspase-inhibitory protein XIAP. In summary, senescent lung fibroblasts induce moderate stress in lung epithelial cells in vitro without affecting growth signaling. |
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Authors:
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Babett Bartling; Grit Rehbein; Rolf-Edgar Silber; Andreas Simm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-04 |
Journal Detail:
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Title: Experimental gerontology Volume: 41 ISSN: 0531-5565 ISO Abbreviation: Exp. Gerontol. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-29 Completed Date: 2007-05-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 532-9 Citation Subset: IM |
Affiliation:
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Department of Cardio-thoracic Surgery, Martin Luther University Halle-Wittenberg, Ernst-Grube-Street 40, 06120 Halle (Saale), Germany. babett.bartling@medizin.uni-halle.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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physiology* Cell Communication / physiology Cell Death / physiology Cell Proliferation Cells, Cultured Coculture Techniques Culture Media, Conditioned Epithelial Cells / metabolism, physiology* Fibroblasts / metabolism, physiology* Humans Lung / cytology*, metabolism Oxidative Stress / physiology Reactive Oxygen Species / metabolism Respiratory Mucosa / cytology Signal Transduction / physiology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Reactive Oxygen Species |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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