Document Detail


Senescence of human skeletal muscle impairs the local inflammatory cytokine response to acute eccentric exercise.
MedLine Citation:
PMID:  15556970     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The impact of aging on the cytokine response of human skeletal muscle to exercise-induced injury remains poorly understood. We enrolled physically active, young (23-35 years old, n=15) and old (66-78 years old, n=15) men to perform 45 min of downhill running (16% descent) at 75% VO2max. Biopsies of vastus lateralis were obtained 24 h before and 72 h after acute eccentric exercise. Transcripts for inflammatory (TNF-alpha, IL-1beta) and anti-inflammatory cytokines (IL-6, TGF-beta1) were quantified by real-time PCR. Before exercise, cytokine transcripts did not differ with age. At old age, exercise induced a blunted accumulation of transcripts encoding the pan-leukocyte surface marker CD18 (young: 10.1-fold increase, P<0.005; old: 4.7-fold increase, P=0.02; young vs. old: P<0.05). In both age groups, CD18 transcript accumulation strongly correlated with TNF-alpha (young, r=0.87, P<0.001; old, r=0.72, P=0.002) and TGF-beta1 transcript accumulation (young, r=0.80, P<0.001; old, r=0.64, P=0.008). At old age, there was no correlation between IL-1beta and CD18 transcript accumulation. Furthermore, exercise induced IL-6 transcript accumulation in young (3.6-fold, P=0.057) but not in old men. Our results suggest that aging impairs the adaptive response of human skeletal muscle to eccentric exercise by differential modulation of a discrete set of inflammatory and anti-inflammatory cytokine genes.
Authors:
Koichiro Hamada; Edouard Vannier; Jennifer M Sacheck; Alice L Witsell; Ronenn Roubenoff
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.     Date:  2004-11-19
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  19     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-28     Completed Date:  2005-09-21     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  264-6     Citation Subset:  IM    
Affiliation:
Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Age Factors
Aged
Aging / blood,  pathology*,  urine
Antigens, CD18 / metabolism
Biological Markers / blood
Biopsy
C-Reactive Protein / metabolism
Cytokines / metabolism*
DNA, Complementary / metabolism
Exercise / physiology*
Humans
Inflammation / blood,  metabolism,  urine
Leg / pathology
Male
Muscle, Skeletal / chemistry,  pathology*
Running / physiology
Grant Support
ID/Acronym/Agency:
AG15797/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD18; 0/Biological Markers; 0/Cytokines; 0/DNA, Complementary; 9007-41-4/C-Reactive Protein

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