| Senescence of human skeletal muscle impairs the local inflammatory cytokine response to acute eccentric exercise. | |
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MedLine Citation:
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PMID: 15556970 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The impact of aging on the cytokine response of human skeletal muscle to exercise-induced injury remains poorly understood. We enrolled physically active, young (23-35 years old, n=15) and old (66-78 years old, n=15) men to perform 45 min of downhill running (16% descent) at 75% VO2max. Biopsies of vastus lateralis were obtained 24 h before and 72 h after acute eccentric exercise. Transcripts for inflammatory (TNF-alpha, IL-1beta) and anti-inflammatory cytokines (IL-6, TGF-beta1) were quantified by real-time PCR. Before exercise, cytokine transcripts did not differ with age. At old age, exercise induced a blunted accumulation of transcripts encoding the pan-leukocyte surface marker CD18 (young: 10.1-fold increase, P<0.005; old: 4.7-fold increase, P=0.02; young vs. old: P<0.05). In both age groups, CD18 transcript accumulation strongly correlated with TNF-alpha (young, r=0.87, P<0.001; old, r=0.72, P=0.002) and TGF-beta1 transcript accumulation (young, r=0.80, P<0.001; old, r=0.64, P=0.008). At old age, there was no correlation between IL-1beta and CD18 transcript accumulation. Furthermore, exercise induced IL-6 transcript accumulation in young (3.6-fold, P=0.057) but not in old men. Our results suggest that aging impairs the adaptive response of human skeletal muscle to eccentric exercise by differential modulation of a discrete set of inflammatory and anti-inflammatory cytokine genes. |
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Authors:
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Koichiro Hamada; Edouard Vannier; Jennifer M Sacheck; Alice L Witsell; Ronenn Roubenoff |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. Date: 2004-11-19 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 19 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-01-28 Completed Date: 2005-09-21 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 264-6 Citation Subset: IM |
Affiliation:
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Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Age Factors Aged Aging / blood, pathology*, urine Antigens, CD18 / metabolism Biological Markers / blood Biopsy C-Reactive Protein / metabolism Cytokines / metabolism* DNA, Complementary / metabolism Exercise / physiology* Humans Inflammation / blood, metabolism, urine Leg / pathology Male Muscle, Skeletal / chemistry, pathology* Running / physiology |
| Grant Support | |
ID/Acronym/Agency:
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AG15797/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD18; 0/Biological Markers; 0/Cytokines; 0/DNA, Complementary; 9007-41-4/C-Reactive Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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