Document Detail


Senescence-associated HSP60 expression in normal human skin fibroblasts.
MedLine Citation:
PMID:  15791579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normal mammalian fibroblasts cultured in vitro undergo a limited number of divisions before entering a senescent phase in which they can be maintained for long periods but cannot be induced to divide. Senescent cells become unresponsive to growth-promoting signals and exhibit senescent cell morphology with flattened and enlarged cell shape. Several chaperones have a direct effect on cellular senescence. HSP60 has been largely studied in our laboratories and it has been associated with uncontrolled cell proliferation in tumor cells. Since senescence is firmly regulated during cell cycle progression, we wanted to investigate HSP60 protein level during cellular senescence. Our data show that HSP60 increases during the initial stage of senescence and that it is localized in cellular compartments, resembling mitochondria. An increase in HSP60 protein amount is associated with a cell cycle slow-down and it may have a role in cell cycle progression.
Authors:
Valentina Di Felice; Nella Ardizzone; Vito Marcianò; Tommaso Bartolotta; Francesco Cappello; Felicia Farina; Giovanni Zummo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology     Volume:  284     ISSN:  1552-4884     ISO Abbreviation:  -     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-06-07     Completed Date:  2005-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101234285     Medline TA:  Anat Rec A Discov Mol Cell Evol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  446-53     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 Wiley-Liss, Inc.
Affiliation:
Dipartimento di Medicina Sperimentale, Sezione di Anatomia Umana Emerico Luna, Università di Palermo, Palermo, Italy. vdfelice@inwind.it
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / metabolism
Cell Aging / physiology*
Cell Cycle / physiology
Cell Division
Cells, Cultured
Chaperonin 60 / metabolism*
Fibroblasts / metabolism*,  ultrastructure
Fluorescent Antibody Technique, Direct
Humans
Immunoenzyme Techniques
Male
Microscopy, Electron, Scanning
Vimentin / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Chaperonin 60; 0/Vimentin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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