Document Detail

Semisynthetic preparation of 1-O-hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor).
MedLine Citation:
PMID:  3245264     Owner:  NLM     Status:  MEDLINE    
1-O-hexadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor, PAF), (1.9 mumol) was prepared from the total lipid extract of the protozoan Tetrahymena pyriformis 9 x 10(7) cells. The procedure involved mild alkaline hydrolysis of the total lipids, followed by acetylation and purification of the product by preparative TLC and HPLC. The yield was 60% with respect to the content of 1-O-alkyl-2-acyl-sn-glyceryl-3-phosphorylcholine in the total lipids, determined after preparative TLC. The alkyl side chain of the semisynthetic PAF was composed of hexadecyl residue. Our product was identified as PAF according to its biological activity, the chromatographic behaviour on TLC and HPLC, the physicochemical properties and the behaviour under treatment with PLA2 and Lipase from Rhizopus arrhizus. The above procedure is proposed as a facile, inexpensive and convenient method.
M E Lekka; D C Tsoukatos; A D Tselepis; V M Kapoulas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zeitschrift für Naturforschung. C, Journal of biosciences     Volume:  43     ISSN:  0939-5075     ISO Abbreviation:  Z. Naturforsch., C, J. Biosci.     Publication Date:    1988 Sep-Oct
Date Detail:
Created Date:  1989-05-26     Completed Date:  1989-05-26     Revised Date:  2009-11-04    
Medline Journal Info:
Nlm Unique ID:  8912155     Medline TA:  Z Naturforsch C     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  665-70     Citation Subset:  IM    
Department of Chemistry, University of Ioannina, Greece.
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MeSH Terms
Blood Platelets / drug effects,  physiology
Chromatography, Gas
Chromatography, High Pressure Liquid / methods
Lipids / isolation & purification
Platelet Activating Factor / chemical synthesis*,  isolation & purification,  pharmacology
Tetrahymena / metabolism
Reg. No./Substance:
0/Lipids; 0/Platelet Activating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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