Document Detail


Seminal LDH-C4 isoenzyme and sperm mitochondrial activity: a study in male partners of infertile couples.
MedLine Citation:
PMID:  14510339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lactate dehydrogenase isoenzyme patterns were obtained in the seman of 93 male partners of infertile couples and 28 proven fertile subjects as a control group. Sperm mitochondrial activity index (SMAI) alongwith the conventional seminal parameters was studied for all the subjects excepting the azoospermic and vasectomised males. Only LDH-C4, a germ cell specific isoenzyme activity varied with the variation in sperm density. LDH-C4 activity varied significantly (p<0.001) within and between different groups. Lactate dehydrogenase-C4 activity was absent in 17 azoospermic samples, confirming its germinal epithelial origin, as well as in 8 samples of vasectomised males. In one azoospermic sample, there were many immature germ cells along with surprisingly high LDH-C4 activity suggesting more activity of germinal epithelium associated with high LDH-C4 activity. LDH-C4 activity was reduced significantly in oligozoospermic samples in proportion to sperm density, thus confirming strong correlation (p<0.001) between LDH-C4 and sperm density. There was statistically significant correlation between LDH-C4 and percentage sperm motility as well as between LDH-C4 and Sperm Mitochondrial Activity Index (SMAI) (probability varying from p<0.05 to p<0.01 in different groups), but no such correlation was found between LDH-C4 and sperm morphology. The data confirms LDH-C4 as a germinal epithelial marker. Its relationship with percentage sperm motility is suggestive of definite role of LDH-C4 in evaluation of the spermatozoal quality, similarly its relationship with Sperm Mitochondrial Activity Index (SMAI score) suggest the role of LDH-C4 in metabolism of the spermatocytes and sperms, though further studies are required for clear and detailed understanding of its metabolic role in semen.
Authors:
M V Sawane; S B Kaore; R D Gaikwad; P M Patil; S S Patankar; A M Deshkar
Related Documents :
16598189 - Male biased sex ratio in the mediterranean fruit fly ceratitis capitata, an example of ...
18477609 - Yuri gagarin is required for actin, tubulin and basal body functions in drosophila sper...
12589659 - Meiosis in male pl/j mice: a genetic model for gametic aneuploidy.
20209439 - Sperm mitochondria diaphorase activity--a gene mapping study of recombinant inbred stra...
7316609 - Histology of accessory sex organs and extragonadal sperm reserves in the male hopping m...
9358009 - Estimation of aneuploidy for chromosomes 3, 7, 16, x and y in spermatozoa from 10 normo...
7577699 - A study of the short tandem repeat systems humvwa and humth01 in an austrian population...
21739569 - Contiguous ∼16 mb 1p36 deletion: dominant features of classical distal 1p36 monosom...
17483479 - Telomere-associated endonuclease-deficient penelope-like retroelements in diverse eukar...
Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article    
Journal Detail:
Title:  Indian journal of medical sciences     Volume:  56     ISSN:  0019-5359     ISO Abbreviation:  Indian J Med Sci     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2003-09-26     Completed Date:  2003-10-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0373023     Medline TA:  Indian J Med Sci     Country:  India    
Other Details:
Languages:  eng     Pagination:  560-6     Citation Subset:  IM    
Affiliation:
Department of Physiology, Government Medical College, Nagpur-440003.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Humans
Infertility, Male / enzymology*
Isoenzymes / metabolism*
L-Lactate Dehydrogenase / metabolism*
Male
Mitochondria / enzymology*
Oligospermia / enzymology
Reference Values
Sperm Count
Sperm Motility / physiology
Spermatozoa / enzymology*
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.1.1.27.-/lactate dehydrogenase C4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of minocycline and tetracycline on immunological responses in experimental animals.
Next Document:  Absolute coronary risk analyser--a tool for managing coronary heart disease risk.