Document Detail

Seminal LDH-C4 isoenzyme and sperm mitochondrial activity: a study in male partners of infertile couples.
MedLine Citation:
PMID:  14510339     Owner:  NLM     Status:  MEDLINE    
Lactate dehydrogenase isoenzyme patterns were obtained in the seman of 93 male partners of infertile couples and 28 proven fertile subjects as a control group. Sperm mitochondrial activity index (SMAI) alongwith the conventional seminal parameters was studied for all the subjects excepting the azoospermic and vasectomised males. Only LDH-C4, a germ cell specific isoenzyme activity varied with the variation in sperm density. LDH-C4 activity varied significantly (p<0.001) within and between different groups. Lactate dehydrogenase-C4 activity was absent in 17 azoospermic samples, confirming its germinal epithelial origin, as well as in 8 samples of vasectomised males. In one azoospermic sample, there were many immature germ cells along with surprisingly high LDH-C4 activity suggesting more activity of germinal epithelium associated with high LDH-C4 activity. LDH-C4 activity was reduced significantly in oligozoospermic samples in proportion to sperm density, thus confirming strong correlation (p<0.001) between LDH-C4 and sperm density. There was statistically significant correlation between LDH-C4 and percentage sperm motility as well as between LDH-C4 and Sperm Mitochondrial Activity Index (SMAI) (probability varying from p<0.05 to p<0.01 in different groups), but no such correlation was found between LDH-C4 and sperm morphology. The data confirms LDH-C4 as a germinal epithelial marker. Its relationship with percentage sperm motility is suggestive of definite role of LDH-C4 in evaluation of the spermatozoal quality, similarly its relationship with Sperm Mitochondrial Activity Index (SMAI score) suggest the role of LDH-C4 in metabolism of the spermatocytes and sperms, though further studies are required for clear and detailed understanding of its metabolic role in semen.
M V Sawane; S B Kaore; R D Gaikwad; P M Patil; S S Patankar; A M Deshkar
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article    
Journal Detail:
Title:  Indian journal of medical sciences     Volume:  56     ISSN:  0019-5359     ISO Abbreviation:  Indian J Med Sci     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2003-09-26     Completed Date:  2003-10-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0373023     Medline TA:  Indian J Med Sci     Country:  India    
Other Details:
Languages:  eng     Pagination:  560-6     Citation Subset:  IM    
Department of Physiology, Government Medical College, Nagpur-440003.
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MeSH Terms
Analysis of Variance
Infertility, Male / enzymology*
Isoenzymes / metabolism*
L-Lactate Dehydrogenase / metabolism*
Mitochondria / enzymology*
Oligospermia / enzymology
Reference Values
Sperm Count
Sperm Motility / physiology
Spermatozoa / enzymology*
Reg. No./Substance:
0/Isoenzymes; EC Dehydrogenase; EC dehydrogenase C4

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