| Seminal LDH-C4 isoenzyme and sperm mitochondrial activity: a study in male partners of infertile couples. | |
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MedLine Citation:
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PMID: 14510339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lactate dehydrogenase isoenzyme patterns were obtained in the seman of 93 male partners of infertile couples and 28 proven fertile subjects as a control group. Sperm mitochondrial activity index (SMAI) alongwith the conventional seminal parameters was studied for all the subjects excepting the azoospermic and vasectomised males. Only LDH-C4, a germ cell specific isoenzyme activity varied with the variation in sperm density. LDH-C4 activity varied significantly (p<0.001) within and between different groups. Lactate dehydrogenase-C4 activity was absent in 17 azoospermic samples, confirming its germinal epithelial origin, as well as in 8 samples of vasectomised males. In one azoospermic sample, there were many immature germ cells along with surprisingly high LDH-C4 activity suggesting more activity of germinal epithelium associated with high LDH-C4 activity. LDH-C4 activity was reduced significantly in oligozoospermic samples in proportion to sperm density, thus confirming strong correlation (p<0.001) between LDH-C4 and sperm density. There was statistically significant correlation between LDH-C4 and percentage sperm motility as well as between LDH-C4 and Sperm Mitochondrial Activity Index (SMAI) (probability varying from p<0.05 to p<0.01 in different groups), but no such correlation was found between LDH-C4 and sperm morphology. The data confirms LDH-C4 as a germinal epithelial marker. Its relationship with percentage sperm motility is suggestive of definite role of LDH-C4 in evaluation of the spermatozoal quality, similarly its relationship with Sperm Mitochondrial Activity Index (SMAI score) suggest the role of LDH-C4 in metabolism of the spermatocytes and sperms, though further studies are required for clear and detailed understanding of its metabolic role in semen. |
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Authors:
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M V Sawane; S B Kaore; R D Gaikwad; P M Patil; S S Patankar; A M Deshkar |
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Publication Detail:
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Type: Clinical Trial; Controlled Clinical Trial; Journal Article |
Journal Detail:
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Title: Indian journal of medical sciences Volume: 56 ISSN: 0019-5359 ISO Abbreviation: Indian J Med Sci Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2003-09-26 Completed Date: 2003-10-23 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0373023 Medline TA: Indian J Med Sci Country: India |
Other Details:
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Languages: eng Pagination: 560-6 Citation Subset: IM |
Affiliation:
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Department of Physiology, Government Medical College, Nagpur-440003. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Humans Infertility, Male / enzymology* Isoenzymes / metabolism* L-Lactate Dehydrogenase / metabolism* Male Mitochondria / enzymology* Oligospermia / enzymology Reference Values Sperm Count Sperm Motility / physiology Spermatozoa / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.1.1.27.-/lactate dehydrogenase C4 |
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