| Semiconservative strand-displacement transcription of the M2 dsRNA segment of Ustilago maydis virus. | |
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MedLine Citation:
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PMID: 2728615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The P1 strain of the Ustilago maydis virus (UmV) is a segmented dsRNA virus with segments designated H1, H2, M1, M2, and L. Incubation of purified virus with a mixture of nucleotides containing 32P-UTP resulted in labeled dsRNA which was retained in the capsid and labeled ssRNA which was released from the capsid. This in vitro transcription reaction was dependent on Mg2+ ion and the optimum concentration for maximum incorporation was 10 mM. The pH and temperature optima were 8.0 and 30 degrees C, respectively. The ssRNA transcripts were precipitated from the supernatant solution of the reaction mixture after ultracentrifugation to separate the virus. Transcription products from supernatant solution hybridized with all five virion dsRNAs. Further studies of the M2 segment indicated that it was labeled within 2 h and the label was completely chased out in 2 h. Analysis of the labeled M2 dsRNA segment by strand-separation gel showed that only one strand (slow moving) was labeled. When both strands were tested in an in vitro translation system, only the slow-moving strand was translated to produce a 24 kDa product. Thus the M2 dsRNA segment of UmV P1 transcribes by a semiconservative strand-displacement mechanism. |
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Authors:
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S W Yie; G K Podila; R F Bozarth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virus research Volume: 12 ISSN: 0168-1702 ISO Abbreviation: Virus Res. Publication Date: 1989 Mar |
Date Detail:
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Created Date: 1989-07-10 Completed Date: 1989-07-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 221-37 Citation Subset: IM |
Affiliation:
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Department of Life Sciences, Indiana State University, Terre Haute 47809. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Basidiomycota
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genetics* Fungal Proteins / genetics Protein Biosynthesis RNA Viruses / genetics* RNA, Double-Stranded / genetics RNA, Viral / genetics* Transcription, Genetic Ustilago / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/RNA, Double-Stranded; 0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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