Document Detail

Semiconductor nanomembrane tubes: three-dimensional confinement for controlled neurite outgrowth.
MedLine Citation:
PMID:  21366271     Owner:  NLM     Status:  MEDLINE    
In many neural culture studies, neurite migration on a flat, open surface does not reflect the three-dimensional (3D) microenvironment in vivo. With that in mind, we fabricated arrays of semiconductor tubes using strained silicon (Si) and germanium (Ge) nanomembranes and employed them as a cell culture substrate for primary cortical neurons. Our experiments show that the SiGe substrate and the tube fabrication process are biologically viable for neuron cells. We also observe that neurons are attracted by the tube topography, even in the absence of adhesion factors, and can be guided to pass through the tubes during outgrowth. Coupled with selective seeding of individual neurons close to the tube opening, growth within a tube can be limited to a single axon. Furthermore, the tube feature resembles the natural myelin, both physically and electrically, and it is possible to control the tube diameter to be close to that of an axon, providing a confined 3D contact with the axon membrane and potentially insulating it from the extracellular solution.
Minrui Yu; Yu Huang; Jason Ballweg; Hyuncheol Shin; Minghuang Huang; Donald E Savage; Max G Lagally; Erik W Dent; Robert H Blick; Justin C Williams
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-03-09
Journal Detail:
Title:  ACS nano     Volume:  5     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-26     Completed Date:  2011-07-28     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2447-57     Citation Subset:  IM    
Department of Electrical and Computer Engineering, University of Wisconsin - Madison, Madison, Wisconsin 53706, USA.
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MeSH Terms
Cell Culture Techniques
Membranes, Artificial*
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Neurons / cytology*
Grant Support
1R01EB009103-01/EB/NIBIB NIH HHS; NS064014/NS/NINDS NIH HHS; R01 EB009103-01/EB/NIBIB NIH HHS; R01 NS064014-03/NS/NINDS NIH HHS
Reg. No./Substance:
0/Membranes, Artificial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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