Document Detail


Semi-quantitative analysis of Ruminococcus flavefaciens, Fibrobacter succinogenes and Streptococcus bovis in the equine large intestine using real-time polymerase chain reaction.
MedLine Citation:
PMID:  18377691     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is a need to further our understanding of the role that the equine hindgut ecosystem plays in digestive processes and diseases. The aim of the present study was to utilise the real-time PCR technique to determine the abundance of candidate cellulolytic (Ruminococcus flavefaciens; Fibrobacter succinogenes) and non-cellulolytic (Streptococcus bovis) bacteria in lumen contents from the caecum, ventral and dorsal colon, and rectum of healthy horses (n 14). Total DNA was extracted from frozen and lyophilised lumen contents, and PCR primers and Taqman probes were designed based on 16S rDNA sequences for specific detection of candidate bacterial species. Overall, in frozen and lyophilised digesta, there were significantly (P F. succinogenes > S. bovis (P < 0.05), while in lyophilised digesta R. flavefaciens was present in significantly (P < 0.05) greater amounts than F. succinogenes and S. bovis. R. flavefaciens and F. succinogenes were abundant at significantly (P < 0.05) greater levels in lyophilised digesta v. frozen digesta, with no difference in S. bovis levels. These data indicate that for these bacteria at least, faeces are a suitable model for studying the bacterial ecosystem within the equine colon. The present study also indicates that the preservation method of digesta affects levels of bacteria detected.
Authors:
Peter M Hastie; Katherine Mitchell; Jo-Anne M D Murray
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-01
Journal Detail:
Title:  The British journal of nutrition     Volume:  100     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-13     Completed Date:  2009-02-26     Revised Date:  2009-05-20    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  561-8     Citation Subset:  IM    
Affiliation:
Division of Veterinary Cell Sciences, Institute of Comparative Medicine, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 1QH, UK. P.Hastie@vet.gla.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Cecum / microbiology
Colon / microbiology
Feces / microbiology
Fibrobacter / genetics*
Horses / microbiology*
Intestine, Large / microbiology*
RNA, Ribosomal, 16S / analysis
Rectum / microbiology
Reverse Transcriptase Polymerase Chain Reaction / methods
Ruminococcus / genetics*
Streptococcus bovis / genetics*
Chemical
Reg. No./Substance:
0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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