Document Detail

Semi-conservative transcription of double-stranded RNA catalyzed by bacteriophage phi 6 RNA polymerase.
MedLine Citation:
PMID:  7462197     Owner:  NLM     Status:  MEDLINE    
Treatment of Pseudomonas phaseolicola double-stranded RNA bacteriophage phi 6 with sodium deoxycholate converted the virions to nucleocapsids, which had in vitro RNA polymerase activity. The incorporation of [3H]UMP continued for at least 7 h. The initial incorporation was detected as intermediate RNA. Radioactivity was chased first into three segments of double-stranded RNA, and then into small, medium, and large species of single-stranded RNA successively via the intermediate RNA. Several copies of single-stranded RNA at least were synthesized from a template. The RNA synthesis clearly took place by a semi-conservative mechanism with respect to templates. That is, 5-bromo UTP was incorporated into one strand of double-stranded RNA to make a hybrid RNA of brominated and unbrominated strands. Furthermore, one strand of the 3H-labeled parental double-stranded RNA was shown to be released as single-stranded RNA.
Y Emori; H Iba; Y Okada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry     Volume:  88     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-04-24     Completed Date:  1981-04-24     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1569-75     Citation Subset:  IM    
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MeSH Terms
Bacteriophages / metabolism*
Capsid / metabolism
DNA-Directed RNA Polymerases / metabolism*
Deoxycholic Acid
RNA, Double-Stranded / biosynthesis*
RNA, Viral / biosynthesis*
Transcription, Genetic*
Uridine Triphosphate / analogs & derivatives
Reg. No./Substance:
0/RNA, Double-Stranded; 0/RNA, Viral; 3398-50-3/5-bromouridine triphosphate; 63-39-8/Uridine Triphosphate; 83-44-3/Deoxycholic Acid; EC RNA Polymerases

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