Document Detail


Self-seal reagent: evaporation control for molecular histology procedures without chambers, clips or fingernail polish.
MedLine Citation:
PMID:  9266090     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sensitive nucleic acid based detection methods such as in situ PCR, in situ RT-PCR and PRINS have great potential in the areas of developmental biology, pathogenesis and diagnostics. However, control of evaporation from in situ reactions is critical to ensure reliable data. Self-Seal Reagent, a component added directly to the in situ reaction mixture, effectively controls evaporation during in situ procedures by creating an evaporation-limiting barrier around the periphery of a standard cover glass as the reaction proceeds. At the end of the procedure, the cover glass is easily removed by soaking in an aqueous solution. A model is presented for how Self-Seal Reagent controls evaporation while maintaining reagent concentrations. Self-Seal Reagent is shown to be effective in the detection of HIV sequences in cells by in situ PCR.
Authors:
D E Sullivan; L E Bobroski; O Bagasra; M Finney
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioTechniques     Volume:  23     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-23     Completed Date:  1997-10-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  320-5     Citation Subset:  IM; X    
Affiliation:
MJ Research, Inc., Watertown, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
Cosmetics
DNA, Viral / analysis
Equipment Design
HIV / genetics
Histocytochemistry
Humans
In Situ Hybridization / instrumentation,  methods*
Polymerase Chain Reaction / instrumentation,  methods*
T-Lymphocytes / virology
Water
Chemical
Reg. No./Substance:
0/Cosmetics; 0/DNA, Viral; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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