|Self-recognition of CD1 by gamma/delta T cells: implications for innate immunity.|
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|PMID: 10727456 Owner: NLM Status: MEDLINE|
|The specificity of immunoglobulins and alpha/beta T cell receptors (TCRs) provides a framework for the molecular basis of antigen recognition. Yet, evolution has preserved a separate lineage of gamma/delta antigen receptors that share characteristics of both immunoglobulins and alpha/beta TCRs but whose antigens remain poorly understood. We now show that T cells of the major tissue gamma/delta T cell subset recognize nonpolymorphic CD1c molecules. These T cells proliferated in response to CD1+ presenter cells, lysed CD1c+ targets, and released T helper type 1 (Th1) cytokines. The CD1c-reactive gamma/delta T cells were cytotoxic and used both perforin- and Fas-mediated cytotoxicity. Moreover, they produced granulysin, an important antimicrobial protein. Recognition of CD1c was TCR mediated, as recognition was transferred by transfection of the gamma/delta TCR. Importantly, all CD1c-reactive gamma/delta T cells express V delta 1 TCRs, the TCR expressed by most tissue gamma/delta T cells. Recognition by this tissue pool of gamma/delta T cells provides the human immune system with the capacity to respond rapidly to nonpolymorphic molecules on professional antigen presenting cells (APCs) in the absence of foreign antigens that may activate or eliminate the APCs. The presence of bactericidal granulysin suggests these cells may directly mediate host defense even before foreign antigen-specific T cells have differentiated.|
|F M Spada; E P Grant; P J Peters; M Sugita; A Melián; D S Leslie; H K Lee; E van Donselaar; D A Hanson; A M Krensky; O Majdic; S A Porcelli; C T Morita; M B Brenner|
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|Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.|
|Title: The Journal of experimental medicine Volume: 191 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 2000 Mar|
|Created Date: 2000-04-04 Completed Date: 2000-04-04 Revised Date: 2010-09-01|
Medline Journal Info:
|Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES|
|Languages: eng Pagination: 937-48 Citation Subset: IM; X|
|Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women's Hospital at Harvard Medical School, Boston, Massachusetts 02115, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Amino Acid Sequence
Anti-Infective Agents / metabolism
Antigen-Presenting Cells / immunology, metabolism
Antigens, CD1 / biosynthesis, metabolism*
Antigens, CD95 / physiology
Antigens, Differentiation, T-Lymphocyte / biosynthesis
Cell Differentiation / immunology
Cytotoxicity Tests, Immunologic
Gene Rearrangement, delta-Chain T-Cell Antigen Receptor
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
Membrane Glycoproteins / physiology
Molecular Sequence Data
Pore Forming Cytotoxic Proteins
Receptors, Antigen, T-Cell, gamma-delta / genetics, metabolism*, physiology
T-Lymphocyte Subsets / cytology, immunology*, metabolism*, microbiology
Th1 Cells / immunology, metabolism
|0/Anti-Infective Agents; 0/Antigens, CD1; 0/Antigens, CD95; 0/Antigens, Differentiation, T-Lymphocyte; 0/GNLY protein, human; 0/Membrane Glycoproteins; 0/Pore Forming Cytotoxic Proteins; 0/Receptors, Antigen, T-Cell, gamma-delta; 126465-35-8/Perforin|
|J Exp Med. 2000 Mar 20;191(6):907-14
Journal ID (nlm-ta): J Exp Med
Publisher: The Rockefeller University Press
© 2000 The Rockefeller University Press
Received Day: 15 Month: 9 Year: 1999
Revision Requested Day: 10 Month: 11 Year: 1999
Accepted Day: 17 Month: 11 Year: 1999
Print publication date: Day: 20 Month: 3 Year: 2000
Volume: 191 Issue: 6
First Page: 937 Last Page: 948
Publisher Id: 991648
PubMed Id: 10727456
|Self-Recognition of Cd1 by γ/δ T Cells : Implications for Innate Immunity|
|Franca M. Spadaa|
|Ethan P. Granta|
|Peter J. Petersb|
|David S. Lesliea|
|Hoi K. Leec|
|Elly van Donselaarb|
|Dennis A. Hansond|
|Alan M. Krenskye|
|Steven A. Porcellia|
|Craig T. Moritac|
|Michael B. Brennera||
Address: Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women's Hospital, 1 Jimmy Fund Way, Boston, MA 02115. 617-525-1010 617-525-1000
aDivision of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women's Hospital at Harvard Medical School, Boston, Massachusetts 02115
bThe Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
cDivision of Rheumatology, Department of Internal Medicine and Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa 52242
dVirginia Mason Research Center, Seattle, Washington 98101
eDivision of Immunology and Transplantation Biology, Department of Pediatrics, Stanford University, Stanford, California 94305
fInstitute of Immunology, University of Vienna, A-1090 Vienna, Austria
Studies in mice and humans have implicated γ/δ T cells in host defense. For example, γ/δ T cells modulate the severity of disease or mediate a component of protective immunity in murine models of listeriosis 1, tuberculosis 234, malaria 5, and HSV-1 encephalitis 6. γ/δ T cells are critically important in preventing death from airway infection with Nocardia asteroides7. In humans, large expansions of γ/δ T cells during infections suggest their importance. γ/δ T cells increase from normal levels of 4% of all circulating T cells to a mean of 12, 14, 29, and 57% of all circulating T cells during infection with Listeria monocytogenes, Mycobacterium tuberculosis, Brucella melitensis, and Ehrlichia chaffeensis, respectively 891011. Despite the growing list of experimental models and human diseases with γ/δ T cell expansions, only a few examples of well-characterized antigen-specific γ/δ T cells have been noted, and these do not support MHC-restricted peptide-specific recognition 1213. Instead, in mice, γ/δ T cell recognition of an intact (unprocessed) cell surface glycoprotein of HSV was noted in one case, whereas in other cases, alloreactive γ/δ T cell recognition of TL and I-E was not dependent on bound peptides 141516. These studies, together with analysis of CDR3 length distribution of the TCR δ chain 17 and the structure of a Vδ domain revealing shared features with both immunoglobulins and α/β TCRs 18, led to the suggestion that, in some cases, γ/δ recognition may be more like that of immunoglobulins than of α/β TCRs.
Major advances in the study of antigen recognition by human γ/δ T cells were made with the discovery that most circulating human γ/δ T cells that bear Vγ2/Vδ2 (Vγ2 is also termed Vγ9) TCRs could be stimulated by nonpeptide antigens (for a review, see reference 19) derived from Mycobacteria2021222324. These nonpeptide antigens were identified as isopentenyl pyrophosphate (IPP) and related prenyl pyrophosphate molecules 2425 and the more recently characterized alkylamine antigens 26. Both the phosphate and the amine antigens are small molecules consisting of short (typically one to five carbons) straight or branched aliphatic chains and either a phosphate or an amine moiety. They are important products of microbes as well as self-antigens. The mechanism by which these antigens are presented is not known, but it does not involve the known MHC class I or MHC class II peptide antigen-presenting molecules 232728, and we have suggested they may be recognized much as haptens are recognized by either immunoglobulins or TCRs 2329. Namely, their requirement for an antigen-presenting element is unclear, but their recognition is critically dependent on the CDR3 sequence of the γ/δ TCR 2930. The recognition of these small aliphatic phosphate and amine organic molecules was exclusively found among γ/δ T cells of the Vδ2 subset.
In contrast to Vγ2/Vδ2+ T cells that are the major circulating pool of γ/δ T cells, human γ/δ T cells bearing Vδ1-encoded TCRs account for the vast majority of γ/δ T cells in tissues such as intestine and spleen 31. Yet, little is known about the antigen reactivity of these tissue γ/δ T cells. Recently, examples of γ/δ T cells in this subset were found to recognize the MHC-encoded proteins, MICA and MICB 32. Recognition was through the activating NKG2D C-type lectin 33 with an unclear contribution from the γ/δ TCR. MICA and MICB class I molecules identify stressed cells and have a very restricted pattern of expression primarily limited to the intestine. MICA and MICB probably do not present peptides, as they have a limited peptide binding groove 34. Instead, these molecules may function in innate immunity as important targets for Vδ1+ γ/δ T cell killing of stressed cells 32.
Here, we provide evidence that an important TCR-mediated reactivity of tissue γ/δ T cells is against CD1 molecules. Remarkably, all of the Vδ1 cells studied were focused specifically on CD1c, one member of a family of nonpolymorphic CD1 molecules, expressed exclusively on APCs, that present lipid and glycolipid foreign antigens to T cells 35. However, the γ/δ T cell lines and clones showed highly specific, direct reactivity to CD1c proteins that was not dependent on the presence of an exogenous foreign antigen. These CD1c-specific γ/δ T cells produced inflammatory cytokines, killed CD1c-bearing targets, and contained bactericidal granulysin.
The following mAbs were used for flow cytometry and blocking experiments: P3 (IgG control) 22, SPV-T3b (anti-CD3) 36, anti–TCR-δ1 (pan anti-Cδ) 37, δTCS1 (anti-Vδ1/Jδ1) 38, TiγA (anti-Vγ2) 3940, 9.3 (anti-CD28) 41, OKT4 (anti-CD4; American Type Culture Collection), OKT8 (anti-CD8α; American Type Culture Collection), DX1 (anti–NKR-P1A; provided by Dr. L. Lanier, DNAX, Palo Alto, CA), BMAO31 (pan anti–TCR-α/β; provided by Dr. R.G. Kurrle, Boehringwerke, Marburg, Germany), 7C6 (anti-CD1c) 42, F10/21A3 (anti-CD1c) 42a, BCD1b3.2 (anti-CD1b) 43, 10H3.9.3 (anti-CD1a) 44, W6/32 (anti–MHC class I; American Type Culture Collection), L243 (anti–HLA-DR; American Type Culture Collection), NS4.1 (IgM control; American Type Culture Collection), 4A11 (anti-Vγ1.4) 45, CD95 Fas ZB4 clone (anti-Fas; Immunotech), δG9 (antiperforin; Ancell), DH2 (antigranulysin) 46, and MPC11 (IgG2b control; American Type Culture Collection).
Cells were incubated with mouse mAbs on ice for 30 min, washed, and stained with FITC-conjugated F(ab′)2 goat anti–mouse Ig (Tago) for an additional 30 min on ice. After washing, the cells were resuspended in propidium iodide and analyzed by flow cytometry (FACSort™; Becton Dickinson). Results were expressed as percentage of positive cells compared with negative cells stained with isotype-matched control mAbs.
Lymphocytes were isolated from the blood of random healthy donors by Ficoll-Hypaque centrifugation. γ/δ T cells were enriched by staining with the anti–TCR-α/β mAb, BMAO31, followed by depletion of α/β T cells with magnetic beads coated with goat anti–mouse IgG (Dynal). T cell lines JR.2 and XV.1 were established from two separate donors by culturing 1 × 106 freshly isolated γ/δ T cells in 1-ml culture wells with 1 × 106 irradiated (5,000 rads) autologous CD1+ monocyte-derived dendritic cells as APCs and the organic phase of a chloroform/methanol/water (2:1:1) extract of M. tuberculosis prepared as described 47 at 1:5,000 final dilution. After 2 wk of culture, viable cells were recovered and the residual α/β T cells were depleted with BMAO31 mAb and magnetic beads (Dynal). The resulting population was restimulated with heterologous CD1+ dendritic cells, an organic phase extract of M. tuberculosis, and rIL-2 (1 nM; Ajinomoto Co.). T cells were maintained by restimulation every 2 wk with irradiated heterologous CD1+ dendritic cells and rIL-2. T cell clones were derived from lines JR.2 and XV.1 by limiting dilution culture using PHA stimulation. T cells were seeded at 1 and 5 cells per well in 96-well round-bottomed plates in a volume of 0.2 ml, with 5 × 104 irradiated (4,000 rads) heterologous PBMCs and 5 × 104 irradiated (5,000 rads) EBV-transformed B lymphoblastoid cells as feeders in RPMI medium supplemented with PHA-P (1:4,000 final dilution; Difco) and IL-2 (2 nM).
Monocyte-derived dendritic cells were generated from human blood monocytes that were isolated from the byproducts of platelet pheresis and induced to differentiate and express CD1a, CD1b, and CD1c by incubation with GM-CSF and IL-4 as described 48. The lymphoblastoid cell lines C1R were transfected with the expression vector pSRα-Neo into which cDNAs encoding either CD1a, CD1b, or CD1c were inserted as described previously 48. HeLa cells were either mock transfected (HeLa Mock) or CD1c transfected (HeLa CD1c) 49.
Cytolytic assays were performed in a 4-h chromium-release assay 43. The targets used were CD1-expressing GM-CSF/IL-4–treated blood monocytes; C1R lymphoblastoid cells either mock transfected (C1R Mock), CD1b transfected (C1R CD1b), or CD1c transfected (C1R CD1c); and HeLa Mock or HeLa CD1c cells 49. In the mAb blocking experiments, targets were incubated with mAbs (20 μg/ml) for 30 min at room temperature before adding effector cells. In experiments where both anti-Fas mAb and strontium ions were used, the targets were incubated for 1 h with anti-Fas mAb (1 μg/ml) before adding the effectors. The effector T cells were treated by incubating with 25 mM strontium chloride hexahydrate (Aldrich Chemical Co.) at 37°C overnight, washing, and then counting after trypan blue incubation to determine their viability. For the M. tuberculosis–specific CD1b-restricted α/β T cell line DN1 47, the target C1R/CD1b was incubated overnight at 37°C with M. tuberculosis total sonicate at 1 μg/ml before T cell addition. For Vγ2/Vδ2 T cell clones specific for IPP and related compounds, the antigen monoethyl phosphate (MEP) was added together with the effectors T cells and targets (1:300) 24. Assays were performed in triplicate, and results were expressed as percent specific 51Cr release ± SEM.
5 × 104 T cells were plated in triplicate in 96-well flat-bottomed plates with either 5 × 104 CD1+ dendritic cells or 5 × 104 mitomycin C–treated HeLa Mock and HeLa CD1c cells as APCs. In the mAb blocking experiments, mAbs were added as ascites (final dilution of 1:200) or purified mAbs (20 μg/ml). Cultures were incubated at 37°C, pulsed with 1 μCi of [3H]thymidine (2 Ci/mmol; Amersham Pharmacia Biotech) on day 2, and harvested 16 h later using a Tomtec harvester. The filter papers were counted on a Betaplate scintillation counter (Wallac). Results were expressed as cpm ± SEM.
5 × 105 T cells from the JR.2 and XV.1 lines were cultured with 5 × 105 CD1+ monocyte-derived dendritic cells in the absence or presence of an anti-CD1c mAb (7C6) or an IgM mAb control (NS4.1). PHA-P (1:4,000) was added as a positive control. Supernatants were harvested after 24 and 48 h of culture. Cytokine release was determined for IL-2, IL-4, IL-10, and IFN-γ by sandwich ELISA assay 50 using antibody pairs purchased from PharMingen (IL-2, IL-4, IL-10) or from Endogen (IFN-γ). Results were expressed as ng/ml ± SEM.
For confocal microscopy analysis, cells were fixed and permeabilized as described 49. For the JR.2 line, cells were incubated with either antiperforin directly conjugated with FITC (δG9 mAb, 1–4 ng/ml) or with antigranulysin (DH2 mAb , 2.5–8 μg/ml) followed by incubation with goat anti–mouse IgG-Cy3 (4 μg/ml; Jackson ImmunoResearch Labs), in single and double staining. Negative controls were MPC11-FITC mAb (IgG2b isotype control for δG9 mAb) together with DH2-Cy3, and P3-Cy3 mAb (IgG1 isotype control for DH2 mAb) together with δG9-FITC. For the 12G12 clone, cells were incubated either with δG9 mAb (3 μg/ml) or with DH2 mAb followed by incubation with anti–mouse Ig–Texas red (2.5–5 μg/ml; Molecular Probes) and goat anti–mouse IgG1-FITC (2.5–5 μg/ml; Southern Biotechnology Associates), respectively, in single and double staining. Controls were δG9–Texas red with P3-FITC and DH2-FITC with MPC11–Texas red, double staining. Each incubation was performed at 4°C for 1 h; in the double staining, cells were incubated for 10 min with 10% mouse serum between the first and second mAb staining.
For electron microscopy, T cells were harvested from culture and dead cells were removed by Ficoll-Hypaque density gradient centrifugation. 0.5–1 × 107 live T cells were fixed at room temperature with 2% glutaraldehyde (Polysciences) in 0.1 M phosphate buffer for 2 h. The fixed cells were then collected into 0.1 M phosphate buffer containing 0.2% paraformaldehyde (Electron Microscopy Sciences) and processed for ultrathin cryosectioning as described 52. Cryosections were incubated with antigranulysin mAb, DH2, for 45 min, washed, and then incubated with protein A–gold (Electron Microscopy Laboratory, Utrecht University, Utrecht, The Netherlands) for 30 min. Labeled sections were viewed with a JOEL 1010 electron microscope at 80 kV.
Total RNA was isolated as described 53 from six JR.2 clones obtained from the JR.2 line. The γ and δ chains were amplified by PCR as described 29 using the following primers: Vδ1 chain, 5′-GGGCTCGAGCTTCAGGCAGCACAACT-3′ (5′ untranslated region [UTR]) and 5′-GGGAGATCTTGGCAGCTCTTTGATGGTGGTTGCTTTGGTTT-3′ (Cδ region); Vγ2 chain, 5′-GGGGTCGACCTGGTGAAGTCATACAGT-3′ (Vγ2 internal region) and 5′-GGGXCTAGAGTGAGGTTCTCTGTGT-3′ (Cγ 3′ UTR).
The PCR products were cloned into pBluescript II (Stratagene), and the sequences of the V-J junctional region were determined using an automated sequencer (PE Applied Biosystems).
Full-length cDNAs encoding the JR.2 TCR were amplified by PCR using the following primers: 5′-GGGCTCGAGCTTCAGGCAGCACAACT-3′ (Vδ1 5′ UTR), 5′-GGGGGATCCGGAGTGTAGCTTCCTCAT-3′ (Vδ1 3′ UTR), 5′-GGGGGTACCTGCCCTGGCAGAAAGCA-3′ (Vγ2 5′ UTR), and 5′-GGGCTCGAGATGGCCTCCTTGTGC-3′ (Vγ2 3′ UTR).
TCR reconstitution was carried out essentially as described 54. The γ and δ cDNAs were cloned into pREP7 and pREP9 (Invitrogen), respectively. J.RT3-T3 cells, Jurkat derivatives that lack cell surface TCR expression 55, were obtained from American Type Culture Collection. J.RT3-T3.5 cells were transfected with 20 μg each of pREP7-JR.2γ and pREP-JR.2δ by electroporation at 250 V, 960 μF with a Gene Pulser (Bio-Rad). Control transfectants were electroporated with 20 μg each of the pREP7 and pREP9 vectors alone. After 48 h, transfectants were placed into medium containing 1 mg/ml geneticin (Life Technologies) and 0.5 mg/ml hygromycin B (Life Technologies). After 2 wk, transfectants were analyzed for cell surface TCR expression by flow cytometry using anti-CD3 (SPV-T3b) and anti-Cδ TCR (anti–TCR-δ1) mAbs and used as responders in T cell stimulation assays. The JR.2/J.RT3 derived in this fashion had 35% TCR-γ/δ+ cells that stained with anti–TCR-δ1 to a mean fluorescence intensity of ∼100 on flow cytometry.
J.RT3-T3.5 transfectants (1 × 105/well) were cultured in 96-well flat-bottomed microtiter plates either in the presence of 10 ng/ml PMA alone, or in the presence of PMA plus CD1+ monocyte-derived dendritic cells with or without the following mAbs (at a concentration of 20 μg/ml): P3 (isotype control), 10H3.9.3 (anti-CD1a), BCD1b3.2 (anti-CD1b), or F10/21A3.1 (anti-CD1c) in a total volume of 200 μl/well. 50 μg aliquots of the culture supernatants were collected after 20–24 h culture at 37°C and diluted 1:2 with culture medium. To measure the amount of IL-2 released into the supernatants, HT-2 cells (5,000/well) were added and cultured 25–30 h at 37°C. [3H]Thymidine (1 μCi/well, 6.7 Ci/mmol; New England Nuclear) was added during the final 5–6 h of culture. Plates were harvested on a Tomtec plate harvester, and [3H]thymidine incorporation was measured in a Betaplate liquid scintillation counter (Wallac).
The limited germline diversity of the γ/δ TCR had led to the suggestion that these cells might recognize nonpolymorphic antigen-presenting molecules 56. Since CD1 molecules resemble MHC but lack polymorphism, we pursued efforts to isolate CD1-restricted γ/δ T cells. To derive γ/δ T cell lines, normal human PBMCs were depleted of α/β T cells by antibody staining and magnetic bead separation. The γ/δ T cell–enriched PBMCs were cultured in vitro with an organic phase extract of M. tuberculosis in the presence of CD1-expressing monocyte-derived dendritic cells (see Materials and Methods). After several restimulations and expansions, two independently derived T cell lines, JR.2 and XV.1, were obtained. Flow cytometric analyses revealed that both lines were of the Vδ1 γ/δ T cell subset. The JR.2 line expressed Vγ2 (mAb TiγA+) and Vδ1/Jδ1 (mAb δTCS1+), whereas the XV.1 line expressed Vγ1.4 (mAb 4A11+) and Vδ1/Jδ1 (mAb δTCS1+). Both cell lines lacked expression of NKR-P1A, CD28, and CD4. As is common for γ/δ T cells 57, 27% (JR.2) and 32% (XV.1) of the cells expressed the CD8 α chain but not the CD8 β chain (Fig. 1, and data not shown).
Although the cell lines were expanded in the presence of M. tuberculosis antigens, the T cells did not specifically recognize M. tuberculosis antigens. Instead, JR.2 cells proliferated in the presence of CD1+ dendritic cells (64,675 cpm), even without M. tuberculosis antigens. This response was not blocked by mAbs against CD1a or CD1b, but was blocked to 307 cpm by mAb against CD1c. Nearly identical results were obtained for XV.1 cells (Fig. 2 A). Addition of a standard M. tuberculosis lipid/lipoglycan extract 47 did not augment proliferation by either line (data not shown). To confirm the specificity suggested by mAb blocking, CD1c transfectant cells were studied. JR.2 γ/δ T cells proliferated markedly to HeLa CD1c 49 but not to HeLa Mock cells (Fig. 2 B). Note that JR.2 T cells did not appear to recognize MICA, as these T cells did not recognize the HeLa Mock cell line that expresses MICA 58.
As it is known that many γ/δ cell lines are capable of cell-mediated cytolysis 13, JR.2 and XV.1 T cells were examined for their ability to lyse transfected C1R lymphoblastoid cell lines C1R CD1b, C1R CD1c, or C1R Mock 35. JR.2 and XV.1 T cells failed to lyse C1R CD1b or C1R Mock targets, but efficiently lysed C1R CD1c targets (70 and 62% specific lysis, respectively; Fig. 2 C). In each case, lysis was specifically blocked (99 and 63% inhibition) by anti-CD1c mAb. Similar results were obtained in cytolytic assays using CD1-expressing monocyte-derived dendritic cells (data not shown). Thus, JR.2 and XV.1 γ/δ T cells were CD1c specific as revealed in the proliferation and cytolytic analyses of CD1 transfectant cell lines and proliferation assays with CD1+ monocyte-derived dendritic cells. Note that these experiments were performed without addition of M. tuberculosis antigens.
To assess the ability to produce cytokines upon exposure to CD1c, cells from the JR.2 line and the XV.1.14 clone were cocultured with CD1-expressing human dendritic cells, and cytokine levels were measured. Substantial levels of IFN-γ (10 and 0.28 ng/ml, respectively) and IL-2 (19 and 17 ng/ml, respectively) were produced after the T cell lines were exposed to CD1+ dendritic cells. Cytokines also were secreted at somewhat higher levels after stimulation with the mitogen PHA (36 and 2.4 ng/ml IFN-γ, and 45 and 9.2 ng/ml IL-2, respectively). The production of these cytokines to CD1c-expressing dendritic cells was blocked completely by the presence of an anti-CD1c mAb (Fig. 3). In contrast to these cytokines, IL-4 and IL-10 were undetectable under these conditions (detection limits were <16 pg/ml IL-4 and <24 pg/ml IL-10 for cytokine ELISA). Thus, the profile of cytokine production by CD1c-reactive γ/δ T cells was Th1-like.
CD1-restricted, microbial-specific CD8+ α/β T cells lyse targets using perforin-mediated pathways and impart a bactericidal effect mediated by granulysin, whereas CD1-restricted CD4−CD8− α/β T cells lyse targets through Fas–FasL interaction 5159. As γ/δ T cells typically display cytolytic potential, we examined the cellular cytotoxicity mechanisms that CD1c-reactive γ/δ T cells used. Fas-dependent lysis can be inhibited by certain anti-Fas mAbs, whereas exhaustive granule secretion induced by strontium ions renders T cells dependent on perforin and granule-related enzymes temporarily incapable of killing target cells 59. To determine the cellular cytotoxicity mechanism of γ/δ T cells, a panel of γ/δ T cells was examined including the JR.2 line and IDP2 clone that are CD1c reactive 60 and previously studied Vγ2/Vδ2-bearing clones, 12G12, DG.SF68, HD.108, and CP.1.15 2557, that are specific for small phosphate antigens such as MEP 24. 12G12 and CP.1.15 are CD8+, whereas HD.108 and DG.SF68 are CD4−CD8−. The antigen-specific lysis of targets by all of these γ/δ T cell clones was consistently and efficiently blocked by treatment with strontium ions (60–84% average inhibition; Fig. 4). Inhibition was observed when cells were treated with anti-Fas mAb as well, but this inhibition was variable. In contrast to γ/δ T cells, the TCR-α/β–expressing CD1b-restricted CD4−CD8− T cell line, DN1 47, lysed antigen-pulsed targets in a manner that was unaffected by pretreatment with strontium ions but was efficiently blocked by mAb against Fas (Fig. 4). These data indicate that cytolysis mediated by both Vδ1+ CD1c-reactive γ/δ T cells and Vδ2+ prenyl pyrophosphate–specific γ/δ T cells can be both perforin mediated and Fas mediated, like that described for CD8+ MHC class I–restricted α/β T cells 61.
Granulysin is a protein present in the cytotoxic granules of cytolytic α/β T cells and NK cells 62 that has potent antimicrobial activity against a variety of bacteria including M. tuberculosis, Staphylococcus aureus, and Listeria monocytogenes51. However, granulysin can kill intracellular bacteria only after the infected cells are lysed by perforin 51. To determine if CD1c-reactive γ/δ T cells express granulysin and therefore had the potential to kill intracellular bacteria, we examined the JR.2 cell line for expression of granulysin and perforin. JR.2 (CD1c-reactive) as well as 12G12 (phosphoantigen-specific) γ/δ T cell lines expressed both granulysin and perforin. These proteins were found to colocalize in cytolytic granules by confocal microscopy (Fig. 5 A). IgG control mAbs showed no significant staining regardless of isotype (data not shown). Further studies by immunoelectron microscopy confirmed the presence of granulysin in the cytolytic granules of both the JR.2 line and the 12G12 clone (Fig. 5 B) as demonstrated by the presence of immunogold particles in cytotoxic granules after staining with an antigranulysin mAb.
Although studies using mAb blocking and transfection of CD1c-encoding expression vectors into recipient targets showed directly that CD1c is the critical molecule recognized by Vδ1+ γ/δ T cells, we sought to determine the role of the TCR in this recognition. To demonstrate that the TCR-γ/δ mediates recognition of CD1c, a series of mAbs blocking experiments and TCR transfection studies was carried out. mAbs against TCR-α/β and TCR-γ/δ were tested for their ability to block JR.2 T cell lysis of C1R CD1c transfectant cells. Only the mAb against the TCR-γ/δ blocked killing (83% inhibition), suggesting that CD1c recognition was TCR mediated (Fig. 6 A). To formally demonstrate that the TCR plays a direct role in the recognition of CD1c, we cloned the TCR γ and δ chains from the CD1c-reactive JR.2 T cell clone by reverse transcription PCR, transfected the JR.2 Vγ2/Vδ1 TCR into the TCR-deficient recipient Jurkat T cell tumor cell line, and measured the ability of the transfectants to produce IL-2 in response to CD1+ dendritic cells. When cultured with CD1+ dendritic cells, the Vγ2/Vδ1 TCR transfectant (JR.2/J.RT3) secreted IL-2 as judged in bioassays by the proliferation of HT-2 cells (34,000 cpm; Fig. 6 B). The production of IL-2 was inhibited completely by adding anti-CD1c mAb, but not anti-CD1a or anti-CD1b mAbs. Mock transfectant J.RT3 recipient cells (Mock/J.RT3) did not produce IL-2 in response to CD1+ presenting cells. Thus, γ/δ T cell specificity for CD1c is determined by the Vδ1-containing TCR, as transfection of this TCR confers reactivity upon the recipient.
The recognition of CD1d antigen-presenting molecules by α/β T cells in the absence of exogenous foreign antigens has been observed for a unique subset of CD4+ mouse NKT cells expressing a canonical Vα14-Jα281 rearrangement 63 as well as for human T cells expressing the homologous Vα24-Jα18 rearrangement 50. The CD1c-reactive γ/δ T cell lines derived here express γ/δ TCRs encoded by Vδ1 gene segments (Fig. 1). To determine the nature of γ/δ TCR diversity involved in recognition of CD1c, we amplified and sequenced the junctional regions of the γ and δ chains of a JR.2 subclone. These sequences were compared with those of γ/δ T cell line IDP2 6465, previously characterized as specific for CD1c 60. Both IDP2 and JR.2 rearranged Vδ1 to Dδ2, Dδ3, and Jδ1 gene segments to encode the junctional region of Vδ1 chain. However the amino acid sequences of the Vδ1 chains in the CDR3 region of the two T cells have almost no homology and are of substantially different lengths. The Vγ2 chains of the clones exhibited limited N region diversity but used different Jγ segments (Jγ1.2 for JR.2 and Jγ2.3 for IDP2; Fig. 7). Vγ usage differed in the XV.1 cell line that used the Vγ1.4 gene segment and another CD1c-reactive Vδ1+ T cell clone 66 that used either Vγ1.3 or Vγ1.4 with Jγ2.3. Thus, CD1c-reactive γ/δ T cells have divergent Vδ1 junctional regions rather than a canonical TCR as found on CD1d-reactive NKT cells and can use different Vγ gene segments. However, since only a limited number of CD1c-reactive TCRs has been analyzed, more TCRs will need to be sequenced to determine the diversity of TCR junctions used by CD1c-reactive γ/δ T cells.
We have described and functionally analyzed γ/δ T cell lines and clones derived from normal donors that directly recognize human CD1c molecules. Compared with other CD1-reactive T cells described in humans, these are remarkable because their recognition was not dependent on the presence of foreign lipid or glycolipid antigens. These CD1c-reactive T cells constitute a subset of directly reactive T cells that may be part of the innate immune system. A certain similarity exists with murine CD4+ α/β NKT cells reactive to murine CD1.1, a homologue of human CD1d 6367. These murine α/β T cells express markers of the NK locus (NK1.1) and express nondiverse, canonical Vα14-Jα281 TCR α chains frequently paired with diverse Vβ8 chains. The production of IL-4 and IFN-γ by these NKT cells has been implicated as an early immunoregulatory response in mice 63686970, and activation of these cells in vivo by exogenous antigen directs conventional T cells to the Th2 phenotype 7172. The secretion of large amounts of IFN-γ in response to exogenous antigen can also inhibit ongoing Th2 responses 69. In contrast, the γ/δ T cells described here are reactive to CD1c, another CD1 family member. Recognition of CD1c is through the γ/δ TCR as directly demonstrated by transfection of the TCR (Fig. 6). TCR γ and δ gene sequences from these T cells revealed the incorporation of template-independent N nucleotides resulting in diverse noncanonical V-(D)-J junctions. Moreover, CD1c-reactive γ/δ T cells lacked expression of NKR-P1A (Fig. 1), a human member of the family of NK receptors analogous to murine NK1.1 73.
It is not clear if the CD1c-reactive γ/δ T cells are responding to CD1c molecules alone or to CD1c molecules containing a self-lipid molecule. The recent findings that insect-derived murine CD1d molecules contain a bound lipid in their antigen binding pocket 74, that mammalian CD1 can bind cellular glycosylphosphatidylinositol 75, and that murine and human Vα14 invariant chain, NKR+ α/β T cells respond to autologous CD1d molecules loaded with glycosylceramides 7677 suggest that the recognition of murine CD1d involves CD1d-bound lipid. Thus, it seems likely that the γ/δ T cells described here are recognizing a self-lipid presented by CD1c. However, such a lipid must be broadly distributed, being present in B cells, HeLa cells, and dendritic cells, as CD1c expressed on these various cell sources was recognized in each case (Fig. 2a and Fig. b).
The recognition of CD1c by human γ/δ T cells may be an important form of antigen recognition by these T cells. Recognition of CD1c by γ/δ T cells in the absence of exogenous foreign antigens could be compared with the alloreactive and autoreactive recognition of MHC class I and class II proteins by α/β T cells that are easily detected in MLRs (mixed lymphocyte reactions) and AMLRs (autologous mixed lymphocyte reactions) 78. Thus, autoreactivity to restricting elements may be a common theme between TCRs that recognize foreign antigens in the context of MHC or CD1.
The best-studied human γ/δ T cell reactivity is to small aliphatic phosphate molecules typically composed of an isoprenoid chain linked to a pyrophosphate moiety 24. Recently, we have characterized another class of aliphatic molecules typically consisting of four or five carbons chains linked to a primary amine 26. Recognition of these small aliphatic compounds may represent a form of pattern recognition by the major circulating γ/δ T cell subset defined by expression of Vδ2 TCR. The Vγ2/Vδ2 subset of γ/δ T cells accounts for the vast majority of human γ/δ T cells in the circulation.
Here, we characterized recognition by the Vδ1 subset of γ/δ T cells, the major population in tissues such as the intestine and the spleen. We suggest that this subset of γ/δ T cells focuses on recognition of nonpolymorphic cell surface molecules related in structure to classical MHC molecules. Groh and Spies have recently demonstrated that some members of this γ/δ T cell subset killed target cells expressing stress-induced MICA and MICB MHC–encoded structures 32 through reactivity with the NKG2D NK receptor 33. Although mAbs specific for the γ/δ TCR blocked γ/δ T cell killing of MICA+ targets 32, the blocking was partial and TCR gene transfer was not done. It remains to be definitely determined if Vδ1+ TCRs can recognize MICA. Here, we show that some members of this same receptor subset recognize nonpolymorphic CD1c. Two other described γ/δ T cell clones reactive with human CD1c also expressed TCRs encoded by the Vδ1 gene segment 6066. Moreover, we have derived and partially characterized two additional Vδ1+ T cell lines that recognize CD1c (data not shown). Thus, these findings suggest that CD1c reactivity may be common among Vδ1+ T cells. However, it is clear that many Vδ1+ T cells are not CD1c reactive (data not shown), and further studies will be needed to determine the frequency of CD1c-reactive γ/δ T cells. Additionally, since the above γ/δ T cell lines were derived by in vitro culture, primary resident CD1c-reactive γ/δ T cells in situ may not be able to respond to CD1c due to anergy.
Direct recognition of CD1c by Vδ1+ T cells may represent a bridge between innate and adaptive immunity in a similar fashion to recognition of CD1d by murine and human NK+ α/β T cells 5063. Such CD1d-reactive α/β T cells are critically required to mediate IL-12–induced tumor immunity in mice 68, can polarize other T cells to a Th2 phenotype when activated by exogenous antigen 7172, and play regulatory roles in listerial infection 79 and Th2 responses 69. We speculate that CD1c-reactive γ/δ T cells also have unique immunological functions. Evidence from in vivo and in vitro experiments has demonstrated that γ/δ T cells play roles complementary to those of conventional α/β T cells in the host defense against infectious agents, in autoimmune diseases, and in tumor surveillance. We demonstrate here that both the CD1c- and the phosphate antigen–reactive γ/δ T cells are cytotoxic and can use the perforin-dependent cytotoxicity pathway. The perforin pathway plays an important role in antiviral immunity, resistance to intracellular bacteria, tumor surveillance, and other immune functions because it is the primary cytotoxic effector mechanism in host defense 61. Further underscoring the potential functional importance of γ/δ T cells in vivo, we show for the first time that γ/δ T cells express granulysin, a potent antimicrobial protein that in conjunction with perforin kills intracellular M. tuberculosis organisms 51. Granulysin also kills a wide variety of microbes in vitro 5162. Moreover, the CD1c-reactive γ/δ T cells possess a Th1-like cytokine profile, producing significant amount of IFN-γ and IL-2.
CD1 molecules can be directly induced on monocytes as they differentiate into dendritic cells in response to GM-CSF or indirectly in response to agents such as bacteria or inflammatory products that induce the secretion of GM-CSF. Such de novo expression of CD1c can be seen on dendritic cells in granulomas induced by Mycobacterium leprae infection in leprosy 80. We speculate that this new expression of CD1c, or stress-induced endogenous lipid antigens presented by CD1c, could then activate tissue Vδ1+ T cells that are specific for CD1c. After their activation, the CD1c-reactive γ/δ T cells could play a role in directing conventional α/β T cells to the acquisition of a Th1 phenotype through their secretion of IFN-γ. The early secretion of cytokines by murine γ/δ T cells during responses to infectious agents provides evidence for such a role 81. These cells, then, could serve an analogous functional role to that proposed for Vα14+NK1.1+ T cells, except CD1c recognition by γ/δ T cells would lead to IFN-γ production rather than to IFN-γ and IL-4 production that is seen with most NK1.1+ T cells. As CD1c-specific γ/δ T cells are cytotoxic and express granulysin, they could also lyse infected dendritic cells via the perforin pathway and kill released intracellular bacteria by secretion of granulysin. In addition, as CD1c is expressed on some circulating B cells and on mantle zone B cells in lymph nodes (although at low levels) 828384, Vδ1+ γ/δ T cells may play a role in T cell–B cell interactions.
Since various professional APCs, including B cells, tissue dendritic cells, activated monocytes, and activated macrophages in humans all express CD1c and since γ/δ T cells recognizing CD1c appear to do so in the absence of foreign antigens before foreign antigen–specific T cells differentiate and expand, upregulation of CD1c could represent an innate danger signal through the activation of CD1c-specific γ/δ T cells. Thus, such γ/δ T cells may play an early role by activating APCs via Th1 cytokines or may play a role in host defense by directly killing microbes via the combination of perforin and granulysin.
Abbreviations used in this paper: IPP, isopentenyl pyrophosphate; MEP, monoethyl phosphate; UTR, untranslated region.
We thank Jack F. Bukowski for helpful suggestions.
This work was supported by the National Institutes of Health (M.B. Brenner, S.A. Porcelli, A.M. Krensky, and C.T. Morita), the Arthritis Foundation (S.A. Porcelli and C.T. Morita), and the American College of Rheumatology (C.T. Morita). A.M. Krensky is a Burroughs Wellcome Scholar in Experimental Therapeutics and the Shelagh Galligan Professor of Pediatrics.
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Keywords: T lymphocytes, T cell antigen receptors γ/δ, CD1, cytolysis, granulysin.
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