| Self-quenching of uranin: Instrument response function for color sensitive photo-detectors. | |
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MedLine Citation:
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PMID: 21331290 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Concentration is a key determining factor in the fluorescence properties of organic fluorophores. We studied self-quenching of disodium fluorescein (uranin) fluorescence in polyvinyl alcohol (PVA) thin films. The concentration dependent changes in brightness and anisotropy were followed by a lifetime decrease. We found that at a concentration of 0.54 M, the lifetime decreases to 7 ps. At a concentration of 0.18 M the lifetime was 10 ps with the relatively high quantum yield of 0.002. In these conditions the fluorescence intensity decay was homogeneous (well approximated by a single lifetime). We realized that such a sample was an ideal fluorescence lifetime standard for spectroscopy and microscopy, and therefore characterized instrument response functions for a time-domain technique. We show that self-quenched uranin enables measurements free of the color effect, making it a superior choice for a lifetime reference over scattered light. |
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Authors:
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Rafal Luchowski; Sushant Sabnis; Mariusz Szabelski; Pabak Sarkar; Sangram Raut; Zygmunt Gryczynski; Julian Borejdo; Piotr Bojarski; Ignacy Gryczynski |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Journal of Luminescence Volume: 130 ISSN: 0022-2313 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2011-2-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101491025 Medline TA: J Luminescence Country: - |
Other Details:
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Languages: ENG Pagination: 2446-2451 Citation Subset: - |
Affiliation:
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Center for Commercialization of Fluorescence Technologies (CCFT), Department of Molecular Biology & Immunology, UNTHSC, Fort Worth, TX 76107, USA. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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R21 CA149897-01//NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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