Document Detail

Self-assembly of fused homo-oligomers to create nanotubes.
MedLine Citation:
PMID:  19031064     Owner:  NLM     Status:  MEDLINE    
The formation of a nanostructure by self-assembly of a peptide or protein building block depends on the ability of the building block to spontaneously assemble into an ordered structure. We first describe a protocol of fusing homo-oligomer proteins with a given three-dimensional (3D) structure to create new building blocks. According to this protocol, a single monomer A that self-assembles with identical copies to create an oligomer A(1) is covalently linked, through a short linker L, to another monomer B that self-assembles with identical copies to create the oligomer B(j). The result is a fused monomer A-L-B, which has the ability to self-assemble into a nanostructure (A-L-B)(k). We control the self-assembly process of A-L-B by mapping the fused building block onto a planar sheet and wrapping the sheet around a cylinder with the target's dimensions. Finally, we validate the created nanotubes by an optimization procedure. We provide examples of two nanotubes in atomistic model details. One of these has experimental data. In principal, such a protocol should enable the creation of a wide variety of potentially useful protein-based nanotubes with control over their physical and chemical properties.
Idit Buch; Chung-Jung Tsai; Haim J Wolfson; Ruth Nussinov
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  474     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-25     Completed Date:  2008-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  117-31     Citation Subset:  IM    
Department of Human Genetics, Sackler Institute of Molecular Medicine, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
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MeSH Terms
Computational Biology / methods*
Models, Molecular*
Nanotechnology / methods*
Nanotubes, Peptide / chemistry*
Peptides / chemistry*
Protein Structure, Quaternary
Proteins / chemistry*
Grant Support
Reg. No./Substance:
0/Nanotubes, Peptide; 0/Peptides; 0/Proteins

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