Document Detail


Self-assembly of SiO2/Gd-DTPA-polyethylenimine nanocomposites as magnetic resonance imaging probes.
MedLine Citation:
PMID:  20352889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Controlled self-assembly of organic/inorganic magnetic hybrid materials have important applications in magnetic resonance imaging (MRI). In this study, a widely used polycation polyethylenimine was conjugated with gadopentetic acid (Gd-DTPA) as a gadolinium bearing polyelectrolyte (Gd-DTPA-PEI). Next, multilayers of Gd-DTPA-PEI were coated on silica nanoparticles through layer-by-layer (LbL) self-assembly with polyanions as monitored by dynamic light scattering, zeta-potential, and scanning electron microscopy. The thickness of the multilayer film was estimated from quartz crystal microbalance based on counting frequency change of each adsorbed layer. The magnetic relaxation of SiO2/(Gd-DTPA-PEl/polyanion), core-shell nanocomposite was tested at 1.5 T magnetic field in a clinical MRI scanner, and a 3-fold increase in T1 relaxivity to 15.1 Gd mM(-1)s(-1) was noticed comparing to Gd-DTPA small molecules. Dextran sulfate was coated as the outermost layer on the nanocomposite for better biocompatibility as verified by in vitro cytotoxicity studies. This formulation provides good signal intensity enhancement of mouse liver in vivo with only 1/25 dose of clinical standard at 30 and 60 minutes after intravenous injection. This sensitive imaging probe with unique core-shell structures may find broad applications in cellular and molecular imaging.
Authors:
Kui Luo; Jing Tian; Gang Liu; Jiayu Sun; Chunchao Xia; Hehan Tang; Ling Lin; Tianxin Miao; Xuna Zhao; Fabao Gao; Qiyong Gong; Bin Song; Xintao Shuai; Hua Ai; Zhongwei Gu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of nanoscience and nanotechnology     Volume:  10     ISSN:  1533-4880     ISO Abbreviation:  J Nanosci Nanotechnol     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-03-31     Completed Date:  2010-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088195     Medline TA:  J Nanosci Nanotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  540-8     Citation Subset:  IM    
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry,  pharmacology
Cell Line, Tumor
Cell Survival
Gadolinium DTPA / analogs & derivatives*,  chemistry,  pharmacology
Humans
Imines / chemistry*,  pharmacology
Liver / drug effects,  metabolism
Magnetic Resonance Imaging / methods*
Materials Testing
Mice
Nanocomposites / administration & dosage,  chemistry*
Nanotechnology / methods
Polyethylenes / chemistry*,  pharmacology
Polymers / chemistry,  pharmacology
Silicon Dioxide / chemistry*,  pharmacology
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Imines; 0/Polyethylenes; 0/Polymers; 0/poly(ethylene imine); 0/polyanions; 7631-86-9/Silicon Dioxide; 80529-93-7/Gadolinium DTPA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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