Document Detail


Self-assembled protein arrays from an ornithodos moubata salivary gland expression library.
MedLine Citation:
PMID:  23140423     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Protein interactions play a critical role in the regulation of many biological events and their study in a high-throughput format has become a key area of proteomic research. Nucleid Acid Programable Protein Arrays (NAPPA) technology allows the construction of protein arrays from cDNA expression libraries in high-throughput cell-free systems to study protein interaction and functions. Tick saliva contains anti-haemostatic, anti-inflammatory and immunosuppressive proteins that counteract the host haemostatic, immune and inflammatory responses allowing the ingestion of host blood and facilitating its infection by the tick-borne pathogens. Identification of such proteins and their functions could help in the selection of antigenic targets for the development of anti-tick and transmission-blocking vaccines. With that aim, we have prepared a cDNA expression library from the salivary glands of Ornithodoros moubata and subsequently produced a self-assembled protein microarray using 480 randomly selected clones from that library. The reproducibility of the array and it representativeness of the tick salivary protein repertoire have been checked demonstrating that it could be a suitable tool for the identification and functional characterization of soft tick salivary molecules. As these molecules are likely involved in blood meal acquisition through the modulation of the host immune and haemostatic responses, this new high-throughput tool could open new avenues for development new therapeutic agents and control strategies against ticks and tick-borne pathogens.
Authors:
Raul Manzano-Román; Veronica Díaz-Martín; Maria González-González; Sergio Matarraz; Angel Francisco Alvarez-Prado; Joshua Labaer; Alberto Orfao; Ricardo Pérez-Sánchez; Manuel Fuentes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-12
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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