Document Detail


Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery.
MedLine Citation:
PMID:  23224057     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
As a versatile gene vector, minicircle DNA (mcDNA) has a great potential for gene therapy. However, some serious challenges remain, such as to effectively deliver mcDNA into targeted cells/tissues and to non-invasively monitor the delivery of the mcDNA. Superparamagnetic iron oxide (SPIO) nanoparticles have been extensively used for both drug/gene delivery and diagnosis. In this study, an MRI visible gene delivery system was developed with a core of SPIO nanocrystals and a shell of biodegradable stearic acid-modified low molecular weight polyethyleneimine (Stearic-LWPEI) via self-assembly. The Stearic-LWPEI-SPIO nanoparticles possess a controlled clustering structure, narrow size distribution and ultrasensitive imaging capacity. Furthermore, the nanoparticle can effectively bind with mcDNA and protect it from enzymatic degradation. In conclusion, the nanoparticle shows synergistic advantages in the effective transfection of mcDNA and non-invasive MRI of gene delivery.
Authors:
Qian Wan; Lisi Xie; Lin Gao; Zhiyong Wang; Xiang Nan; Hulong Lei; Xiaojing Long; Zhi-Ying Chen; Cheng-Yi He; Gang Liu; Xin Liu; Bensheng Qiu
Related Documents :
23792917 - Adoption of the q transcriptional regulatory system for zebrafish transgenesis.
24709397 - Comparative analysis of internal ribosomal entry sites as molecular tools for bicistron...
24391757 - Peg3 mutational effects on reproduction and placenta-specific gene families.
24121567 - Association of molecular markers derived from the brcristo1 gene with prolycopene-enric...
7768447 - Genetic analysis of ethylene signal transduction in arabidopsis thaliana: five novel mu...
16709197 - Rfi2, a ring-domain zinc finger protein, negatively regulates constans expression and p...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-10
Journal Detail:
Title:  Nanoscale     Volume:  -     ISSN:  2040-3372     ISO Abbreviation:  Nanoscale     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zy.wang@siat.ac.cn bs.qiu@siat.ac.cn.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Implications of maternal vitamin D deficiency for the fetus, the neonate and the young infant.
Next Document:  Preterm Birth and the Shape of Your Heart.