| Self-assembled magnetic filter for highly efficient immunomagnetic separation. | |
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MedLine Citation:
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PMID: 20949198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed a compact and inexpensive microfluidic chip, the self-assembled magnetic filter, to efficiently remove magnetically tagged cells from suspension. The self-assembled magnetic filter consists of a microfluidic channel built directly above a self-assembled NdFeB magnet. Micrometre-sized grains of NdFeB assemble to form alternating magnetic dipoles, creating a magnetic field with a very strong magnitude B (from the material) and field gradient ▽B (from the configuration) in the microfluidic channel. The magnetic force imparted on magnetic beads is measured to be comparable to state-of-the-art microfabricated magnets, allowing for efficient separations to be performed in a compact, simple device. The efficiency of the magnetic filter is characterized by sorting non-magnetic (polystyrene) beads from magnetic beads (iron oxide). The filter enriches the population of non-magnetic beads to magnetic beads by a factor of >10(5) with a recovery rate of 90% at 1 mL h(-1). The utility of the magnetic filter is demonstrated with a microfluidic device that sorts tumor cells from leukocytes using negative immunomagnetic selection, and concentrates the tumor cells on an integrated membrane filter for optical detection. |
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Authors:
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David Issadore; Huilin Shao; Jaehoon Chung; Andita Newton; Mikael Pittet; Ralph Weissleder; Hakho Lee |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural Date: 2010-10-14 |
Journal Detail:
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Title: Lab on a chip Volume: 11 ISSN: 1473-0189 ISO Abbreviation: Lab Chip Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-14 Completed Date: 2011-05-05 Revised Date: 2011-07-19 |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 147-51 Citation Subset: IM |
Affiliation:
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Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor Equipment Design Humans Immunomagnetic Separation / economics, instrumentation* Leukocytes / cytology Mice Microfluidic Analytical Techniques / economics, instrumentation* |
| Grant Support | |
ID/Acronym/Agency:
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2R01-EB004626/EB/NIBIB NIH HHS; R01 EB004626-06/EB/NIBIB NIH HHS; R01 EB004626-07/EB/NIBIB NIH HHS; T32 CA079443-09/CA/NCI NIH HHS; T32-CA79443/CA/NCI NIH HHS; U01 HL080731-04/HL/NHLBI NIH HHS; U01-HL080731/HL/NHLBI NIH HHS; U54 CA119349-05S10003/CA/NCI NIH HHS; U54-CA119349/CA/NCI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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