Document Detail

Self-acylation properties of type II fatty acid biosynthesis acyl carrier protein.
MedLine Citation:
PMID:  17656314     Owner:  NLM     Status:  MEDLINE    
Acyl carrier protein (ACP) plays a central role in many metabolic processes inside the cell, and almost 4% of the total enzymes inside the cell require it as a cofactor. Here, we report self-acylation properties in ACPs from Plasmodium falciparum and Brassica napus that are essential components of type II fatty acid biosynthesis (FAS II), disproving the existing notion that this phenomenon is restricted only to ACPs involved in polyketide biosynthesis. We also provide strong evidence to suggest that catalytic self-acylation is intrinsic to the individual ACP. Mutational analysis of these ACPs revealed the key residue(s) involved in this phenomenon. We also demonstrate that these FAS II ACPs exhibit a high degree of selectivity for self-acylation employing only dicarboxylic acids as substrates. A plausible mechanism for the self-acylation reaction is also proposed.
Ashish Misra; Shailendra Kumar Sharma; Namita Surolia; Avadhesha Surolia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry & biology     Volume:  14     ISSN:  1074-5521     ISO Abbreviation:  Chem. Biol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-27     Completed Date:  2007-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  775-83     Citation Subset:  IM    
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
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MeSH Terms
Acyl Carrier Protein / chemistry,  genetics,  metabolism*
Amino Acid Sequence
Brassica / metabolism
Cloning, Molecular
Fatty Acids / biosynthesis*
Molecular Sequence Data
Mutagenesis, Site-Directed
Plasmodium falciparum / metabolism
Sequence Homology, Amino Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Reg. No./Substance:
0/Acyl Carrier Protein; 0/Fatty Acids

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