Document Detail


Self-Assembly of Folate onto Polyethyleneimine Coated CdS/ZnS Quantum Dots for Targeted Turn-On Fluorescence Imaging of Folate Receptor Over-Expressed Cancer Cells.
MedLine Citation:
PMID:  23194289     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Folate receptor (FR) can be over-expressed by a number of epithelial-derived tumors, but minimally expressed in normal tissues origin. As folic acid (FA) is a high affinity ligand to FR, and not produced endogenously, development of FA conjugated probes for targeted imaging FR over-expressed cancer cells is of significance for assessing cancer therapeutics and for better understanding the expression profile of FR in cancer. Here we report a novel turn-on fluorescence probe for imaging FR over-expressed cancer cells. The probe was easily fabricated via electrostatic self-assembly of FA and polyethyleneimine-coated CdS/ZnS quantum dots (PEI-CdS/ZnS QDs). The primary fluorescence of PEI-CdS/ZnS QDs turned off firstly upon the electrostatic adsorption of FA onto PEI-CdS/ZnS QDs based on electron transfer to produce negligible fluorescence background. The presence of FR expressed on the surface of cancer cells then made FA desorb from PEI-CdS/ZnS QDs due to specific and high affinity of FA to FR. As a result, the primary fluorescence of PEI-CdS/ZnS QDs adhering to the cells turned on due to the inhibition of electron transfer. The most important merits of the developed probe are its simplicity and the effective avoidance of the false positive results due to the simple electrostatic self-assembly of FA onto the surface of PEI-CdS/ZnS QDs and the involved fluorescence "off-on" mechanism. The probe was demonstrated to be sensitive and selective for targeted imaging of FR over-expressed cancer cells in turn-on mode.
Authors:
Yi Zhang; Jing-Min Liu; Xiu-Ping Yan
Related Documents :
18992149 - Leiomyosarcoma of the breast in a patient with a 10-year-history of cyclophosphamide ex...
10960839 - Evaluation of prostatic specific antigen and digital rectal examination as screening te...
1377979 - Prostate cancer screening: current trends and future implications.
12028299 - Does transurethral resection of the prostate facilitate detection of clinically signifi...
20608139 - Prostate specific antigen through the years.
11763389 - Lichen planus pigmentosus-inversus.
8599859 - Promising new agents in the treatment of non-small cell lung cancer.
22155859 - Oncological outcome of laparoscopic surgery for advanced colon cancer: a community hosp...
24626779 - Cu2-xse@msio2-peg core-shell nanoparticles: a low-toxic and efficient difunctional nano...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-30
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Conversion of residual organics in corn stover-derived biorefinery stream to bioenergy via microbial...
Next Document:  Graphitic Design: Prospects of Graphene-Based Nanocomposites for Solar Energy Conversion, Storage, a...