| Self-assembled micronanoplexes for improved biolistic delivery of nucleic acids. | |
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MedLine Citation:
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PMID: 19754152 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method for biolistic delivery of nucleic acids using a combination of cationic micro- and nanoparticles is reported. The new method is simpler to perform than the conventional calcium/spermidine-based formulations and shows 11-fold improved nucleic acid binding capacity and dose-dependent performance both for in vitro and in vivo applications relative to either the conventional preparation or our recently reported direct cationic microparticle method. These features may enable higher throughput gene delivery and genetic immunization programs and open new venues for the biolistic delivery method. |
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Authors:
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Sergei A Svarovsky; Maria J Gonzalez-Moa; Mark D Robida; Alexandre Y Borovkov; Kathryn Sykes |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Molecular pharmaceutics Volume: 6 ISSN: 1543-8392 ISO Abbreviation: Mol. Pharm. Publication Date: 2009 Nov-Dec |
Date Detail:
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Created Date: 2009-12-07 Completed Date: 2010-02-24 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101197791 Medline TA: Mol Pharm Country: United States |
Other Details:
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Languages: eng Pagination: 1927-33 Citation Subset: IM |
Affiliation:
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Center for Innovations in Medicine at the Biodesign Institute, and the School of Life Sciences, Arizona State University, 1001 South McAllister Avenue, Tempe, Arizona 85287-5901, USA. sergei.svarovsky@asu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biolistics / methods* DNA / administration & dosage, chemistry Gold Metal Nanoparticles / chemistry*, ultrastructure* Mice Microscopy, Electron, Transmission Models, Theoretical NIH 3T3 Cells Nucleic Acids / administration & dosage*, chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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AI057156/AI/NIAID NIH HHS; U54 AI057156-05S10031/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 7440-57-5/Gold; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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