| Selenium modification of nucleic acids: preparation of phosphoroselenoate derivatives for crystallographic phasing of nucleic acid structures. | |
| | |
MedLine Citation:
|
PMID: 17406625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This protocol describes a simplified means of introducing an anomalously scattering atom into oligonucleotides by conventional solid-phase synthesis. Replacement of a nonbridging phosphate oxygen in the backbone with selenium is practically suitable for any nucleic acid. The resulting oligonucleotide P-diastereomers can be separated using anion exchange HPLC to yield diastereomerically pure phosphoroselenoates (PSes). The total time for the synthesis and ion-exchange HPLC separation of pure PSe is approximately 60 h. |
| | |
Authors:
|
Pradeep S Pallan; Martin Egli |
Related Documents
:
|
9519765 - A shannon entropy analysis of immunoglobulin and t cell receptor. 5969285 - Analysis of bases of rat-liver nucleic acids after administration of the carcinogen dim... 17443885 - Rna: prebiotic product, or biotic invention? 13475615 - A cytochemical study of the l. e. bodies of systemic lupus erythematosus. i. nucleic ac... 3821385 - Biological effects of fish oils in relation to chronic diseases. 3957585 - Regional pulmonary distribution of iodine-125-labeled oleic acid. its relationship to t... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Nature protocols Volume: 2 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2007 |
Date Detail:
|
Created Date: 2007-04-04 Completed Date: 2007-06-28 Revised Date: 2008-03-24 |
Medline Journal Info:
|
Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
|
Languages: eng Pagination: 640-6 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, High Pressure Liquid Crystallography / methods* Molecular Structure Nucleic Acids / chemistry* Phosphorus Compounds / chemical synthesis*, chemistry Selenium / chemistry* Selenium Compounds / chemical synthesis*, chemistry |
| Grant Support | |
ID/Acronym/Agency:
|
R01 GM55237/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Nucleic Acids; 0/Phosphorus Compounds; 0/Selenium Compounds; 7782-49-2/Selenium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The use of the Ugi four-component condensation.
Next Document: Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2'-SeMe-ur...