| Selenium-enriched milk proteins and selenium yeast affect selenoprotein activity and expression differently in mouse colon. | |
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MedLine Citation:
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PMID: 20346189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Certain forms of dietary Se may have an advantage in improving Se status and reducing cancer risk. The present study compared the effects of an Se-enriched milk protein product (dairy-Se) with an Se yeast (yeast-Se) on selenoprotein activity and expression in the mouse colon. Mice were fed four diets for 4 weeks: a control milk protein diet (Se at 0.068 parts per million (ppm)), dairy-Se diets with Se at 0.5 and 1 ppm, and a yeast-Se diet with Se at 1 ppm. Cytosolic glutathione peroxidase-1 (GPx-1) activity, mRNA of selenoprotein P (SeP), GPx-1, gastrointestinal glutathione peroxidase-2 (GPx-2) and thioredoxin reductase-1 (TrxR-1) were examined in the mouse colon. Dairy-Se diets did not significantly affect GPx-1 mRNA and GPx-1 activity but produced a dose-dependent increase in SeP and GPx-2 mRNA, with a significantly higher level achieved at 1 ppm Se (P < 0.05). Yeast-Se at 1 ppm significantly increased GPx-1 mRNA and GPx-1 activity (P < 0.01) but not GPx-2 mRNA. Neither Se supplement had any effect on TrxR-1. The present study indicates that selenoprotein levels in the mouse colon are regulated differently depending on the Se supplement. As we have previously shown that dairy-Se at 1 ppm was protective against colorectal cancer (CRC) in an azoxymethane-induced CRC mouse model, this up-regulation of colonic GPx-2 and SeP with Se supplementation may be crucial to its chemopreventive action. |
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Authors:
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Ying Hu; Graeme H McIntosh; Richard K Le Leu; Graeme P Young |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-29 |
Journal Detail:
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Title: The British journal of nutrition Volume: 104 ISSN: 1475-2662 ISO Abbreviation: Br. J. Nutr. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-07-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: England |
Other Details:
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Languages: eng Pagination: 17-23 Citation Subset: IM |
Affiliation:
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Flinders Centre for Cancer Prevention and Control, Flinders University of South Australia, Bedford Park 5042, Australia. ying.hu@flinders.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Colon / drug effects*, metabolism Diet Dietary Proteins / pharmacology* Dose-Response Relationship, Drug Food, Fortified Glutathione Peroxidase / genetics, metabolism* Male Mice Mice, Inbred C57BL Milk Proteins / pharmacology* Neoplasms / prevention & control RNA, Messenger / metabolism Selenium / pharmacology* Selenoprotein P / genetics, metabolism* Thioredoxin Reductase 1 / metabolism Yeast, Dried* |
| Chemical | |
Reg. No./Substance:
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0/Dietary Proteins; 0/Milk Proteins; 0/RNA, Messenger; 0/Selenoprotein P; 7782-49-2/Selenium; EC 1.11.1.9/Glutathione Peroxidase; EC 1.8.1.9/Thioredoxin Reductase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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