Document Detail


Selenium and GPx-1 overexpression protect mammalian cells against UV-induced DNA damage.
MedLine Citation:
PMID:  17625244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Supplementation of the culture media of human MCF-7 breast carcinoma cells or mouse fibroblasts with low levels of selenium (30 nM) provided as sodium selenite was shown to protect these cells from ultraviolet (UV)-induced chromosome damage, as quantified by micronucleus assay. Selenium supplementation was also effective in reducing UV-induced gene mutations as measured in the lacI shuttle vector model. Protection was dependent on functional BRCA1 activity, a protein implicated in breast cancer risk and DNA damage repair. In addition, overexpression of GPx-1, a selenoprotein with antioxidant activity, also attenuated UV induced micronuclei formation in the absence of selenium supplementation. Combining selenium supplementation with GPx-1 overexpression further reduced UV-induced micronucleus frequency. These data provide evidence that the benefits of selenium supplementation might be either through the prevention or repair of DNA damage, and they implicate at least one selenoprotein (GPx-1) in the process.
Authors:
Manjeshwar S Baliga; Hengbing Wang; Pin Zhuo; Jeffrey L Schwartz; Alan M Diamond
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Biological trace element research     Volume:  115     ISSN:  0163-4984     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-07-12     Completed Date:  2007-08-15     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  227-42     Citation Subset:  IM    
Affiliation:
Department of Human Nutrition, University of Illinois at Chicago, Chicago, IL 60612, USA.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / etiology,  genetics
Cell Line, Tumor
Cell Survival
DNA Damage*
DNA Repair
Dietary Supplements
Gene Expression Regulation, Enzymologic*
Glutathione Peroxidase / metabolism*
Humans
Micronucleus Tests
Mutagenesis
Risk
Selenium / metabolism*,  pharmacology
Sodium Selenite / pharmacology
Ultraviolet Rays
Grant Support
ID/Acronym/Agency:
5R01CA101053-03/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
10102-18-8/Sodium Selenite; 7782-49-2/Selenium; EC 1.11.1.9/Glutathione Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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