Document Detail


Selectivity of interferon action in simian virus 40-transformed cells superinfected with simian virus 40.
MedLine Citation:
PMID:  2983099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment of African green monkey kidney CV-1 cells with human alpha interferons before infection with simian virus 40 (SV40) inhibited the accumulation of SV40 mRNAs and SV40 T-antigen (Tag). This inhibition persisted as long as the interferons were present in the medium. SV40-transformed human SV80 cells and mouse SV3T3-38 cells express Tag, and interferon treatment of these cells did not affect this expression. SV80 and SV3T3-38 cells which had been exposed to interferons were infected with a viable SV40 deletion mutant (SV40 dl1263) that codes for a truncated Tag. Exposure to interferons inhibited the accumulation of the truncated Tag (specified by the infecting virus) but had no significant effect on the accumulation of the endogenous Tag (specified by the SV40 DNA integrated into the cellular genome). The level of Tag in SV40-transformed mouse SV101 cells was not significantly decreased by interferon treatment. SV40 was rescued from SV101 cells and used to infect interferon-treated and control African green monkey kidney Vero cells. Tag accumulation was inhibited in the cells which had been treated with interferons before infection. Our data demonstrate that even within the same cell the interferon system can discriminate between expression of a gene in the SV40 viral genome and expression of the same gene integrated into a host chromosome.
Authors:
M A García-Blanco; P K Ghosh; B M Jayaram; S Ivory; P Lebowitz; P Lengyel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  53     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-04-03     Completed Date:  1985-04-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  893-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Transformation, Viral*
Cercopithecus aethiops
Genes, Viral
Humans
Interferon Type I / pharmacology*
Mice
RNA, Messenger / analysis
Simian virus 40 / drug effects*,  genetics
Virus Replication / drug effects
Grant Support
ID/Acronym/Agency:
AI 12320/AI/NIAID NIH HHS; CA 16038/CA/NCI NIH HHS; CA 32799/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Interferon Type I; 0/RNA, Messenger
Comments/Corrections

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