| Selectivity for D-lactate incorporation into the peptidoglycan precursors of Lactobacillus plantarum: role of Aad, a VanX-like D-alanyl-D-alanine dipeptidase. | |
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MedLine Citation:
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PMID: 17400741 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lactobacillus plantarum produces peptidoglycan precursors ending in D-lactate instead of D-alanine, making the bacterium intrinsically resistant to vancomycin. The ligase Ddl of L. plantarum plays a central role in this specificity by synthesizing D-alanyl-D-lactate depsipeptides that are added to the precursor peptide chain by the enzyme MurF. Here we show that L. plantarum also encodes a D-Ala-D-Ala dipeptidase, Aad, which eliminates D-alanyl-D-alanine dipeptides that are produced by the Ddl ligase, thereby preventing their incorporation into the precursors. Although D-alanine-ended precursors can be incorporated into the cell wall, inactivation of Aad failed to suppress growth defects of L. plantarum mutants deficient in d-lactate-ended precursor synthesis. |
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Authors:
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Marie Deghorain; Philippe Goffin; Laetitia Fontaine; Jean-Luc Mainardi; Richard Daniel; Jeff Errington; Bernard Hallet; Pascal Hols |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-30 |
Journal Detail:
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Title: Journal of bacteriology Volume: 189 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-05-17 Completed Date: 2007-09-06 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 4332-7 Citation Subset: IM |
Affiliation:
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Unité de Génétique, Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism,
physiology Biological Transport / drug effects Dipeptidases / genetics, metabolism Lactic Acid / metabolism* Lactobacillus plantarum / enzymology, genetics, metabolism* Microscopy, Fluorescence Models, Biological Peptidoglycan / metabolism* Vancomycin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Peptidoglycan; 1404-90-6/Vancomycin; 50-21-5/Lactic Acid; EC 3.4.13.-/Dipeptidases; EC 3.4.13.11/dipeptidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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