Document Detail

A selectively terminable transgenic rice line expressing human lactoferrin.
MedLine Citation:
PMID:  20433928     Owner:  NLM     Status:  MEDLINE    
Human lactoferrin (hLF) is a multifunctional milk protein which could be utilized for promoting human health. Transgenic rice has been used as a bioreactor for mass production of recombinant hLF. However, one major concern over such transgenic rice is the risk of its unintended spreading into environment and into our food supplies. Here we report the development of selectively terminable transgenic rice expressing human lactoferrin in seeds. These transgenic rice plants could be selectively terminated by bentazon, a common herbicide used for rice weed control. The hLF expression cassette was constructed into a T-DNA containing the RNA interference cassette suppressing the expression of the rice gene CYP81A6 which detoxifies herbicide bentazon, and the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) cassette which confers to glyphosate tolerance. A transgenic line, named as G281, was identified for its high sensitivity to bentazon, high tolerance to glyphosate, and high expression of hLF. Southern analysis suggested G281 is a single copy insertion event. Field tests demonstrated that G281 plants can be completely killed by a single spray of bentazon at 1000 mg/L, which is safe to regular rice and represents only half of the dose recommended by manufacturer for rice field weed control. Therefore, any G281 contaminations in regular rice could be selectively terminated to make sure it will not enter food or feed supplies.
Chaoyang Lin; Peng Nie; Wei Lu; Qing Zhang; Jing Li; Zhicheng Shen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-28
Journal Detail:
Title:  Protein expression and purification     Volume:  74     ISSN:  1096-0279     ISO Abbreviation:  Protein Expr. Purif.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9101496     Medline TA:  Protein Expr Purif     Country:  United States    
Other Details:
Languages:  eng     Pagination:  60-4     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
State Key Laboratory of Rice Biology and Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China.
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MeSH Terms
Benzothiadiazines / pharmacology
Gene Expression
Glycine / analogs & derivatives,  pharmacology
Herbicides / pharmacology
Lactoferrin / genetics*
Oryza sativa / drug effects,  genetics*
Plants, Genetically Modified / genetics*
Seeds / genetics
Reg. No./Substance:
0/Benzothiadiazines; 0/Herbicides; 0/Lactoferrin; 1071-83-6/glyphosate; 25057-89-0/bentazone; 56-40-6/Glycine

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