| Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish. | |
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MedLine Citation:
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PMID: 20729204 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The regulation and function of lysosomal hydrolases during yolk consumption and embryogenesis in zebrafish are poorly understood. In an effort to better define the lysosomal biochemistry of this organism, we analyzed the developmental expression, biochemical properties, and function of several glycosidases in zebrafish eggs, embryos, and adult tissues. Our results demonstrated that the specific activity of most enzymes increases during embryogenesis, likely reflecting a greater need for turnover within the embryo as yolk-derived nutrients are depleted. Analysis of glycosidase activity in zebrafish and medaka eggs revealed selective deposition of enzymes required for the degradation of N-linked glycans, including an abundance of acidic mannosidases. Treatment of zebrafish embryos with the α-mannosidase inhibitor swainsonine resulted in the accumulation of glycosylated vitellogenin fragments and demonstrated a function for maternally deposited acid α-mannosidase in yolk consumption. Surprisingly, we also found that, unlike mammals, acid α-glucosidase from zebrafish and medaka does not appear to be modified with mannose 6-phosphate residues. We further showed these residues were not acquired on human acid α-glucosidase when expressed in zebrafish embryos, suggesting unique differences in the ability of the human and zebrafish N-acetylglucosamine-1-phosphotransferase to recognize and modify certain lysosomal glycosidases. Together, these results provide novel insight into the role of acidic glycosidases during yolk utilization and the evolution of the mannose 6-phosphate targeting system in vertebrates. |
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Authors:
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Xiang Fan; Maximilian Klein; Heather R Flanagan-Steet; Richard Steet |
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Publication Detail:
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Type: Journal Article Date: 2010-08-20 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 285 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2010-11-24 Revised Date: 2012-02-21 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 32946-53 Citation Subset: IM |
Affiliation:
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Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Enzyme Inhibitors / pharmacology Glycoside Hydrolases / genetics, metabolism* Humans Lysosomes / enzymology*, genetics Mannose / genetics, metabolism* Mannosephosphates / genetics, metabolism Phosphorylation / drug effects, physiology Swainsonine / pharmacology Yolk Sac / embryology, metabolism* Zebrafish / embryology, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM086524-02/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Mannosephosphates; 31103-86-3/Mannose; 3672-15-9/mannose-6-phosphate; 72741-87-8/Swainsonine; EC 3.2.1.-/Glycoside Hydrolases |
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