Document Detail


Selective vitrification of euploid oocytes markedly improves survival, fertilization and pregnancy-generating potential.
MedLine Citation:
PMID:  18854107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enthusiasm for oocyte cryopreservation has been limited by poor pregnancy rates per thawed metaphase II (MII) oocytes (<4%) and low implantation rates per embryos. The reasons relate to technical limitations in the freezing process, and the fact that <40% of oocytes are euploid and unable to produce 'competent' embryos. Comparative genomic hybridization was performed on the first polar body (PB-1) of 323 MII oocytes retrieved from 16 donors. Of these, 111 were euploid, and were vitrified. Seventy-five of 78 vitrified oocytes (96%) survived warming and were fertilized using intracytoplasmic sperm injection. Thirty-one (41%) subsequently developed into expanded blastocysts, of which no more than two were subsequently transferred per uterus to 16 out of 19 prospective embryo recipients. Twelve of 19 (63%) recipients produced 17 healthy babies (eight singletons, three twins, and one set of triplets) One twin pregnancy miscarried in the late first trimester The birth rate per transfer of a maximum of two blastocysts to 16 recipients was 75%. The implantation rate per vitrified euploid oocyte was 27%. This study showed a six-fold improvement in pregnancy rate per cryopreserved oocyte over previous reports and a marked improvement in implantation rate. If independently validated, this approach could open the door to commercial egg cryobanking, significantly expanding women's reproductive choices.
Authors:
G Sher; L Keskintepe; T Mukaida; M Keskintepe; M Ginsburg; Y Agca; G Maassarani; A Bayrak
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  17     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-15     Completed Date:  2009-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  524-9     Citation Subset:  IM    
Affiliation:
2Department of Obstetrics and Gynecology, Sher Institutes for Reproductive Medicine, University of Nevada School of Medicine, Reno, NV 89109 , USA. gsher@sherinstitute.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Survival
Cryopreservation / methods*
Embryo Transfer
Female
Fertilization / physiology*
Humans
Middle Aged
Oocytes*
Ploidies*
Pregnancy
Pregnancy Rate*
Pregnancy, Multiple / statistics & numerical data
Twins
Young Adult

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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