| Selective vitrification of euploid oocytes markedly improves survival, fertilization and pregnancy-generating potential. | |
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MedLine Citation:
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PMID: 18854107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Enthusiasm for oocyte cryopreservation has been limited by poor pregnancy rates per thawed metaphase II (MII) oocytes (<4%) and low implantation rates per embryos. The reasons relate to technical limitations in the freezing process, and the fact that <40% of oocytes are euploid and unable to produce 'competent' embryos. Comparative genomic hybridization was performed on the first polar body (PB-1) of 323 MII oocytes retrieved from 16 donors. Of these, 111 were euploid, and were vitrified. Seventy-five of 78 vitrified oocytes (96%) survived warming and were fertilized using intracytoplasmic sperm injection. Thirty-one (41%) subsequently developed into expanded blastocysts, of which no more than two were subsequently transferred per uterus to 16 out of 19 prospective embryo recipients. Twelve of 19 (63%) recipients produced 17 healthy babies (eight singletons, three twins, and one set of triplets) One twin pregnancy miscarried in the late first trimester The birth rate per transfer of a maximum of two blastocysts to 16 recipients was 75%. The implantation rate per vitrified euploid oocyte was 27%. This study showed a six-fold improvement in pregnancy rate per cryopreserved oocyte over previous reports and a marked improvement in implantation rate. If independently validated, this approach could open the door to commercial egg cryobanking, significantly expanding women's reproductive choices. |
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Authors:
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G Sher; L Keskintepe; T Mukaida; M Keskintepe; M Ginsburg; Y Agca; G Maassarani; A Bayrak |
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Publication Detail:
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Type: Clinical Trial; Journal Article |
Journal Detail:
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Title: Reproductive biomedicine online Volume: 17 ISSN: 1472-6491 ISO Abbreviation: Reprod. Biomed. Online Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-15 Completed Date: 2009-01-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101122473 Medline TA: Reprod Biomed Online Country: England |
Other Details:
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Languages: eng Pagination: 524-9 Citation Subset: IM |
Affiliation:
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2Department of Obstetrics and Gynecology, Sher Institutes for Reproductive Medicine, University of Nevada School of Medicine, Reno, NV 89109 , USA. gsher@sherinstitute.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cell Survival Cryopreservation / methods* Embryo Transfer Female Fertilization / physiology* Humans Middle Aged Oocytes* Ploidies* Pregnancy Pregnancy Rate* Pregnancy, Multiple / statistics & numerical data Twins Young Adult |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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