| Selective trafficking of non-cell-autonomous proteins mediated by NtNCAPP1. | |
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MedLine Citation:
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PMID: 12532017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In plants, cell-to-cell communication is mediated by plasmodesmata and involves the trafficking of non-cell-autonomous proteins (NCAPs). A component in this pathway, Nicotiana tabacum NON-CELL-AUTONOMOUS PATHWAY PROTEIN1 (NtNCAPP1), was affinity purified and cloned. Protein overlay assays and in vivo studies showed that NtNCAPP1 is located on the endoplasmic reticulum at the cell periphery and displays specificity in its interaction with NCAPs. Deletion of the NtNCAPP1 amino-terminal transmembrane domain produced a dominant-negative mutant that blocked the trafficking of specific NCAPs. Transgenic tobacco plants expressing this mutant form of NtNCAPP1 and plants in which the NtNCAPP1 gene was silenced were compromised in their ability to regulate leaf and floral development. These results support a model in which NCAP delivery to plasmodesmata is both selective and regulated. |
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Authors:
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Jung-Youn Lee; Byung-Chun Yoo; Maria R Rojas; Natalia Gomez-Ospina; L Andrew Staehelin; William J Lucas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 299 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-01-17 Completed Date: 2003-02-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 392-6 Citation Subset: IM |
Affiliation:
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Section of Plant Biology, Division of Biological Sciences, University of California, 1 Shields Avenue, Davis, CA 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Cell Communication Cell Line Cloning, Molecular Cytoplasm / metabolism Endoplasmic Reticulum / metabolism Flowers / growth & development Gene Silencing Green Fluorescent Proteins Immunohistochemistry Luminescent Proteins / metabolism Molecular Sequence Data Mutation Phenotype Plant Leaves / growth & development Plant Proteins / chemistry, genetics, isolation & purification*, metabolism* Plants, Genetically Modified Plasmodesmata / metabolism* Protein Transport Recombinant Fusion Proteins / metabolism Tobacco / genetics, growth & development, metabolism* Tobacco Mosaic Virus Viral Proteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM18639/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/PP16 protein, Cucurbita maxima; 0/Plant Proteins; 0/Recombinant Fusion Proteins; 0/Viral Proteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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