| Selective toxicity of ginsenoside Rg3 on multidrug resistant cells by membrane fluidity modulation. | |
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MedLine Citation:
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PMID: 18365686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Multidrug resistance (MDR) is a major problem in cancer chemotherapy. It was previously reported that a red ginseng saponin, 20(S)-ginsenoside Rg3 could modulate MDR in vitro and extend the survival of mice implanted with ADR-resistant murine leukemia P388 cells. This study examined the cytotoxicity of Rg3 on normal and transformed cells, along with its effect on the membrane fluidity. The cytotoxicity study revealed that 120 microM of Rg3 was cytotoxic against a multidrug-resistant human fibroblast carcinoma cell line, KB V20C, but not against normal WI 38 cells in vitro. Flow cytometric analysis using rhodamine 123 as the artificial substrate showed that Rg3 promoted the accumulation of rhodamine 123 in ADR-resistant murine leukemia P388 cells in vivo. Fluorescence polarization studies using the hydrophilic fluorescent probe, DPH, and hydrophobic probe, TMA-DPH, showed that 20 microM Rg3 induced a significant increase in fluorescence anisotropy in KB V20C cells but not in the parental KB cells. These results clearly show that Rg3 decreases the membrane fluidity thereby blocking drug efflux. |
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Authors:
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Hyog-Young Kwon; Eun-Hye Kim; Seung-Whan Kim; Su-Nam Kim; Jong-Dae Park; Dong-Kwon Rhee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of pharmacal research Volume: 31 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-03-27 Completed Date: 2008-05-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 171-7 Citation Subset: IM |
Affiliation:
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College of Pharmacy, SungKyunKwan University, Su-Won 440-746, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiotics, Antineoplastic / pharmacology Antineoplastic Agents, Phytogenic / toxicity* Doxorubicin / pharmacology Drug Resistance, Multiple / drug effects* Drug Resistance, Neoplasm / drug effects* Female Flow Cytometry Fluorescence Polarization Fluorescent Dyes Ginsenosides / toxicity* Humans KB Cells Leukemia P388 / drug therapy Membrane Fluidity / drug effects* Mice Neoplasm Transplantation Rhodamine 123 |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Antineoplastic Agents, Phytogenic; 0/Fluorescent Dyes; 0/Ginsenosides; 14197-60-5/ginsenoside Rg3; 23214-92-8/Doxorubicin; 62669-70-9/Rhodamine 123 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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