Document Detail


Selective suppression of an endothelin and platelet-derived growth factor containing vesicular system in endothelium of rat saphenous vein by long-term orthostasis.
MedLine Citation:
PMID:  15767762     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electron-dense vesicles were observed in rat vascular endothelium. The purpose of this study was to characterize their content(s), venous-arterial distribution and response to chronic orthostatic stress in extremity vessels. Saphenous and brachial vessels - saphenous vein (SV), saphenous artery (SA), brachial vein, brachial artery - were prepared for electron microscopy to quantitate the vesicle area within the endothelium following immunohistochemical and immunocytochemical identification. The effect of long-term orthostasis was assessed by exposure to head-up tilt for 2 weeks. The vesicular area in relation to the total cross-sectional area of the endothelial cells in the SV and SA of normal and confined control groups was 3.88 +/- 0.38 versus 0.89 +/- 0.06% (p < 0.05) and 4.92 +/- 0.25 versus 1.09 +/- 0.47% (p < 0.05), respectively. Head-up tilt suppressed the vesicle content of the SV to 2.26 +/- 0.39% (p < 0.05), but it remained low in the SA (1.29 +/- 0.45%), brachial vein (0.45 +/- 0.12%) and brachial artery (0.59 +/- 0.17%). Endothelin and platelet-derived growth factor, but not acidic phosphatase activity or lipid content, could be identified in the vesicles. Plasma endothelin levels were unchanged. We conclude that dense vesicles in the endothelium of extremity vessels are not cell degradation products. They may represent a vesicular secretory or storage system for endothelin and platelet-derived growth factor which participates in regional vascular adaptation to long-term orthostatic load.
Authors:
Gábor Raffai; Erzsébet Fehér; Gyorgy Nádasy; Sándor Paku; Gábor Pogány; Ferenc Tímár; Béla Szende; Emil Monos
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-03-14
Journal Detail:
Title:  Journal of vascular research     Volume:  42     ISSN:  1018-1172     ISO Abbreviation:  J. Vasc. Res.     Publication Date:    2005 Mar-Apr
Date Detail:
Created Date:  2005-04-15     Completed Date:  2005-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Copyright Information:
Copyright 2005 S. Karger AG, Basel.
Affiliation:
Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, POB 448, HU-1446 Budapest, Hungary. raffai@elet2.sote.hu
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MeSH Terms
Descriptor/Qualifier:
Animals
Dizziness / metabolism*
Endothelins / antagonists & inhibitors*
Endothelium, Vascular / metabolism*,  ultrastructure
Gravitation
Head-Down Tilt
Immunohistochemistry
Male
Microscopy, Electron
Platelet-Derived Growth Factor / antagonists & inhibitors*
Rats
Rats, Sprague-Dawley
Saphenous Vein / metabolism*,  ultrastructure
Time Factors
Chemical
Reg. No./Substance:
0/Endothelins; 0/Platelet-Derived Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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