Document Detail

Selective staining of Y chromosomal loops in Drosophila hydei, D. neohydei, and D. eohydei.
MedLine Citation:
PMID:  67013     Owner:  NLM     Status:  MEDLINE    
Modified Giemsa procedures have been developed which elicit differential and highly selective staining of individual Y chromosomal lampbruch loops in spermatocyte nuclei of Drosophila hydei, D. NEOHYDEI, AND D. eohydei. In all three species the Y loop pair known as the "clubs" stains a brilliant dark red with Giemsa at pH 10. With the same treatment other loop pairs either remain unstained, e.g. the "threads", or show a differentiation between light blue and pink staining material, e.g. "pseudonucleolus" and "cones" in D. hydei and D. eohydei. With eosin at pH 2.8 the "threads" in D. hydei can be stained intensely, as well as one matrical component of th "pseudonucleolus". Pretreatment with RNase or TCA removes all stainability from the Y loops with Giemsa at Ph 10. TCA treatment enhances eosin staining at pH 2.8. These and other variations of Giemsa may be utilized to establish homologies between Y loops in different species. The molecular basis of the staining reactions remains to be elucidated.
N Yamasaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  60     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  27-37     Citation Subset:  IM    
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MeSH Terms
Azure Stains
Hydrogen-Ion Concentration
Ribonucleoproteins / analysis
Sex Chromosomes / analysis*
Spermatocytes / ultrastructure
Staining and Labeling
Reg. No./Substance:
0/Azure Stains; 0/Fluoresceins; 0/Ribonucleoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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