Document Detail


Selective solid-phase extraction of cholesterol using molecularly imprinted polymers and its application in different biological samples.
MedLine Citation:
PMID:  16859856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Non-covalent molecularly imprinted polymers (MIPs) of cholesterol were prepared by UV initiated polymerization. A polymer that had the highest binding selectivity and capability was used as solid-phase extraction (SPE) sorbents for direct extraction of cholesterol from different biological samples (human serum, cow milk, yolk, shrimp, pork and beef). The extraction conditions of molecularly imprinted SPE (MISPE) were optimized and the optimum protocol was: conditioning MISPE cartridges with n-hexane, loading with n-hexane, washing with n-hexane and n-hexane:toluene=9:1, respectively, then eluting with chloroform:ethanol:acetic acid=3:1:1. Cholesterol MISPE selectively recognized, effectively trapped and pre-concentrated cholesterol over a concentration range of 10-80 microg/mL. Recoveries ranged from 80.6% to 92.7%, with R.S.D. lower than 9.8%. Under the optimal condition, MISPE recoveries of spiked human serum, yolk, cow milk, shrimp, pork and beef were 91.1%, 80.4%, 86.6%, 78.2%, 81.4% and 80.1%, respectively. Compared with C18 SPE, almost all of the matrix interferences were removed after MISPE, and better baselines and higher selectivity were achieved.
Authors:
Yun Shi; Jiang-Hua Zhang; Dan Shi; Ming Jiang; Ye-Xiang Zhu; Su-Rong Mei; Yi-Kai Zhou; Kang Dai; Bin Lu
Related Documents :
3986996 - Gas-chromatographic determination of cholesterol in serum: candidate reference method.
23521496 - Rapid detection of mycobacterium tuberculosis using a capillary-array microsystem with ...
10563786 - An improved method for the microscale preparation and characterization of hapten-protei...
6640896 - Quantification of high-density-lipoprotein cholesterol by precipitation with phosphotun...
12741606 - Computer-assisted method development and optimization in high-performance liquid chroma...
2592516 - Simultaneous determination of biogenic amines, their precursors and metabolites in a si...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-21
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  42     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-31     Completed Date:  2007-02-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  549-55     Citation Subset:  IM    
Affiliation:
MOE Key Laboratory of Environment & Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cholesterol / analysis*,  blood
Egg Yolk / chemistry
Food Analysis / methods*
Humans
Meat / analysis
Milk / chemistry
Molecular Structure
Penaeidae / chemistry
Polymers / chemistry*
Sensitivity and Specificity
Solid Phase Extraction / methods*
Swine
Chemical
Reg. No./Substance:
0/Polymers; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Exploration of linear modelling techniques and their combination with multivariate adaptive regressi...
Next Document:  Development and validation of a simple HPLC method for simultaneous in vitro determination of amoxic...