Document Detail

Selective solid-phase extraction of cholesterol using molecularly imprinted polymers and its application in different biological samples.
MedLine Citation:
PMID:  16859856     Owner:  NLM     Status:  MEDLINE    
Non-covalent molecularly imprinted polymers (MIPs) of cholesterol were prepared by UV initiated polymerization. A polymer that had the highest binding selectivity and capability was used as solid-phase extraction (SPE) sorbents for direct extraction of cholesterol from different biological samples (human serum, cow milk, yolk, shrimp, pork and beef). The extraction conditions of molecularly imprinted SPE (MISPE) were optimized and the optimum protocol was: conditioning MISPE cartridges with n-hexane, loading with n-hexane, washing with n-hexane and n-hexane:toluene=9:1, respectively, then eluting with chloroform:ethanol:acetic acid=3:1:1. Cholesterol MISPE selectively recognized, effectively trapped and pre-concentrated cholesterol over a concentration range of 10-80 microg/mL. Recoveries ranged from 80.6% to 92.7%, with R.S.D. lower than 9.8%. Under the optimal condition, MISPE recoveries of spiked human serum, yolk, cow milk, shrimp, pork and beef were 91.1%, 80.4%, 86.6%, 78.2%, 81.4% and 80.1%, respectively. Compared with C18 SPE, almost all of the matrix interferences were removed after MISPE, and better baselines and higher selectivity were achieved.
Yun Shi; Jiang-Hua Zhang; Dan Shi; Ming Jiang; Ye-Xiang Zhu; Su-Rong Mei; Yi-Kai Zhou; Kang Dai; Bin Lu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-21
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  42     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-31     Completed Date:  2007-02-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  549-55     Citation Subset:  IM    
MOE Key Laboratory of Environment & Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, PR China.
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MeSH Terms
Cholesterol / analysis*,  blood
Egg Yolk / chemistry
Food Analysis / methods*
Meat / analysis
Milk / chemistry
Molecular Structure
Penaeidae / chemistry
Polymers / chemistry*
Sensitivity and Specificity
Solid Phase Extraction / methods*
Reg. No./Substance:
0/Polymers; 57-88-5/Cholesterol

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