| Selective and site-specific mobilization of dermal dendritic cells and Langerhans cells by Th1- and Th2-polarizing adjuvants. | |
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MedLine Citation:
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PMID: 20404167 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dendritic cells (DCs) initiate and polarize adaptive immune responses toward varying functional outcomes. By means of intravital two-photon microscopy, we report that dermal dendritic cells (DDCs) and Langerhans cells (LCs) are differentially mobilized during contact sensitization and by adjuvants such as unmethylated CpG oligonucleotide (CpG) and LPS that induce T helper type 1 (Th1) responses, or papain that induces T helper type 2 (Th2) responses. In ear pinna, contact sensitization, CpG, LPS, and papain all mobilized DDCs in three distinct phases: increased motility and dendritic probing, directed migration, and entry into lymphatic vessels. During the same treatments, the adjacent LCs in ear pinna remained immotile over a 48-hr period of observation. In contrast, footpads lacked DDCs and Th1-polarizing adjuvants selectively induced a delayed mobilization of LCs after 48 hr. Th1 polarization of CD4(+) T cells was independent of the immunization site, whereas ear immunization favored Th2 polarization, correlating with site-specific DC distribution and dynamics. Our results provide an initial description of peripheral DC dynamics in response to adjuvants and imply that LC mobilization enhances a Th1 response and is not sufficient to trigger a Th2 response, whereas mobilization of DDCs alone is sufficient to trigger T-cell proliferation and to polarize initial T-cell activation toward a Th2 response. |
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Authors:
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Debasish Sen; Luette Forrest; Thomas B Kepler; Ian Parker; Michael D Cahalan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-04-19 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-05 Completed Date: 2010-06-08 Revised Date: 2010-11-05 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 8334-9 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics and Institute for Immunology, University of California, Irvine, CA 92697-4561, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement Cell Polarity* Cell Proliferation Coculture Techniques Langerhans Cells / cytology, immunology* Lipopolysaccharides / immunology Lymphocyte Activation Mice Mice, Inbred C57BL Oligodeoxyribonucleotides / immunology Th1 Cells / cytology*, immunology* Th2 Cells / cytology*, immunology* |
| Grant Support | |
ID/Acronym/Agency:
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GM-41514/GM/NIGMS NIH HHS; GM-48071/GM/NIGMS NIH HHS; N01-AI-50019/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CPG-oligonucleotide; 0/Lipopolysaccharides; 0/Oligodeoxyribonucleotides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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