| Selective regulation of arterial branching morphogenesis by synectin. | |
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MedLine Citation:
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PMID: 16740480 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Branching morphogenesis is a key process in the formation of vascular networks. To date, little is known regarding the molecular events regulating this process. We investigated the involvement of synectin in this process. In zebrafish embryos, synectin knockdown resulted in a hypoplastic dorsal aorta and hypobranched, stunted, and thin intersomitic vessels due to impaired migration and proliferation of angioblasts and arterial endothelial cells while not affecting venous development. Synectin(-/-) mice demonstrated decreased body and organ size, reduced numbers of arteries, and an altered pattern of arterial branching in multiple vascular beds while the venous system remained normal. Murine synectin(-/-) primary arterial, but not venous, endothelial cells showed decreased in vitro tube formation, migration, and proliferation and impaired polarization due to abnormal localization of activated Rac1. We conclude that synectin is involved in selective regulation of arterial, but not venous, growth and branching morphogenesis and that Rac1 plays an important role in this process. |
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Authors:
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Thomas W Chittenden; Filip Claes; Anthony A Lanahan; Monica Autiero; Robert T Palac; Eugene V Tkachenko; Arye Elfenbein; Carmen Ruiz de Almodovar; Eduard Dedkov; Robert Tomanek; Weiming Li; Michael Westmore; Jai Pal Singh; Arie Horowitz; Mary Jo Mulligan-Kehoe; Karen L Moodie; Zhen W Zhuang; Peter Carmeliet; Michael Simons |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental cell Volume: 10 ISSN: 1534-5807 ISO Abbreviation: Dev. Cell Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-02 Completed Date: 2006-07-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 101120028 Medline TA: Dev Cell Country: United States |
Other Details:
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Languages: eng Pagination: 783-95 Citation Subset: IM |
Affiliation:
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Angiogenesis Research Center, Section of Cardiology, Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arteries / abnormalities, cytology, embryology*, growth & development* Carrier Proteins / chemistry, genetics, metabolism Cell Movement Cell Proliferation Cells, Cultured Embryo, Nonmammalian Endothelial Cells / cytology, physiology Endothelium, Vascular / cytology Female Femoral Artery / cytology Gene Expression Regulation Gene Expression Regulation, Developmental Mice Mice, Knockout Morphogenesis* Myocardium / cytology Neuropeptides / deficiency*, genetics Pregnancy Venae Cavae / cytology Zebrafish / embryology* Zebrafish Proteins / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HL067960/HL/NHLBI NIH HHS; HL53793/HL/NHLBI NIH HHS; HL62289/HL/NHLBI NIH HHS; T32 AR007576/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Neuropeptides; 0/Rgs19ip1 protein, mouse; 0/Zebrafish Proteins; 0/synectin protein, zebrafish |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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