Document Detail

Selective recovery of target cells employing a bi-functional aptamer.
MedLine Citation:
PMID:  18776577     Owner:  NLM     Status:  In-Process    
Aptamers are single-stranded nucleic acids with high and specific affinity to a target molecule. Recently, target cell-binding aptamers are studied as molecular tools for the detection and the isolation of desired cells. Here, I developed a method for the selective recovery of target cells employing a bi-functional aptamer. As a model experiment, I constructed the bi-functional aptamer by fusing a resin-binding aptamer and a target cell-binding aptamer. The bi-functional aptamer enabled the selective recovery of the target cells simply by mixing of the cells with the bi-functional aptamer and the resin. This simple method would be applied for a variety of cell types and resins.
Shoij P Ohuchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids symposium series (2004)     Volume:  -     ISSN:  1746-8272     ISO Abbreviation:  Nucleic Acids Symp Ser (Oxf)     Publication Date:  2008  
Date Detail:
Created Date:  2008-09-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101259965     Medline TA:  Nucleic Acids Symp Ser (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  709-10     Citation Subset:  IM    
Department of Basic Medical Sciences, IMSUT, The University of Tokyo, Minato-ku, Tokyo 108-0071, Japan.
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