| Selective protein transport: identity of the solubilized phosvitin receptor from chicken oocytes. | |
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MedLine Citation:
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PMID: 6264234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By two independent methods, the solubilized receptor for phosvitin (PV) has a subunit MW of 116K. Affinity chromatography, showed that only 2 of the more than 25 proteins present in the total detergent solubilized oocyte membrane extract were retained on a PV-agarose column. These proteins of MW of 116K and 100K could be eluted from PV-agarose with free PV. By gel exclusion chromatography, the receptor-125I-PV complexes elute in the void volume of a Biogel A-1.5 column. When these void fractions were assayed gy SDS-PAGE only a single protein of MW of 116K was observed in addition to 125I-PV. |
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Authors:
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J W Woods; T F Roth |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of supramolecular structure Volume: 14 ISSN: 0091-7419 ISO Abbreviation: J. Supramol. Struct. Publication Date: 1980 |
Date Detail:
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Created Date: 1981-08-20 Completed Date: 1981-08-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0330464 Medline TA: J Supramol Struct Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 473-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Cell Membrane / analysis Chickens Chromatography, Affinity Chromatography, Gel Egg Proteins* Electrophoresis, Polyacrylamide Gel Female Octoxynol Oocytes / analysis* Ovum / analysis* Polyethylene Glycols Receptors, Cell Surface / isolation & purification* Solubility |
| Grant Support | |
ID/Acronym/Agency:
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HD 09549/HD/NICHD NIH HHS; HD 11519/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Egg Proteins; 0/Polyethylene Glycols; 0/Receptors, Cell Surface; 0/vitellogenin receptor; 9002-93-1/Octoxynol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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