| Selective protein adsorption on polymer patterns formed by self-organization and soft lithography. | |
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MedLine Citation:
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PMID: 19586049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thin films, with both isotropic and ordered patterns of polymer domains, are used as substrates to study selective adsorption of two proteins (concanavalin A and lentil lectin) and to test reconstruction of polymer patterns by these proteins. Integral geometry approach is used to compare quantitatively fluorescence micrographs of protein patches with AFM images of original isotropic patterns, formed during blend casting of polystyrene/poly(methyl methacrylate) and PS/poly(ethylene oxide). Preferential adsorption of both lectins to PMMA phase domains, enhanced for PS/PMMA interfaces is concluded. In turn, protein binding to PS phase regions of PS/PEO blends is highly selective. Ordered protein grouping is obtained as a result of selective adsorption to alternating stripes of polystyrene (partly brominated to enable identification) and cross-linked PEO, prepared with solvent-assisted micromolding applied to PBrS/PEO bilayers. Biological activity test, performed with concanavalin A, confirms preserved functionality of a complementary protein, carboxypeptidase Y, adsorbed to polymer patterns. |
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Authors:
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Joanna Zemła; Małgorzata Lekka; Joanna Raczkowska; Andrzej Bernasik; Jakub Rysz; Andrzej Budkowski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomacromolecules Volume: 10 ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-09-02 Completed Date: 2009-11-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 2101-9 Citation Subset: IM |
Affiliation:
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Smoluchowski Institute of Physics, Jagiellonian University, 30-059 Krakow, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Carboxypeptidase C / metabolism* Concanavalin A / chemistry, metabolism* Microscopy, Atomic Force Plant Lectins / chemistry, metabolism* Polymers / chemistry* Polymethyl Methacrylate / chemistry* Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Plant Lectins; 0/Polymers; 0/lentil lectin; 11028-71-0/Concanavalin A; 9011-14-7/Polymethyl Methacrylate; EC 3.4.16.5/Carboxypeptidase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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