Document Detail


Selective protein adsorption on polymer patterns formed by self-organization and soft lithography.
MedLine Citation:
PMID:  19586049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thin films, with both isotropic and ordered patterns of polymer domains, are used as substrates to study selective adsorption of two proteins (concanavalin A and lentil lectin) and to test reconstruction of polymer patterns by these proteins. Integral geometry approach is used to compare quantitatively fluorescence micrographs of protein patches with AFM images of original isotropic patterns, formed during blend casting of polystyrene/poly(methyl methacrylate) and PS/poly(ethylene oxide). Preferential adsorption of both lectins to PMMA phase domains, enhanced for PS/PMMA interfaces is concluded. In turn, protein binding to PS phase regions of PS/PEO blends is highly selective. Ordered protein grouping is obtained as a result of selective adsorption to alternating stripes of polystyrene (partly brominated to enable identification) and cross-linked PEO, prepared with solvent-assisted micromolding applied to PBrS/PEO bilayers. Biological activity test, performed with concanavalin A, confirms preserved functionality of a complementary protein, carboxypeptidase Y, adsorbed to polymer patterns.
Authors:
Joanna Zemła; Małgorzata Lekka; Joanna Raczkowska; Andrzej Bernasik; Jakub Rysz; Andrzej Budkowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  10     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2009-11-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2101-9     Citation Subset:  IM    
Affiliation:
Smoluchowski Institute of Physics, Jagiellonian University, 30-059 Krakow, Poland.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Carboxypeptidase C / metabolism*
Concanavalin A / chemistry,  metabolism*
Microscopy, Atomic Force
Plant Lectins / chemistry,  metabolism*
Polymers / chemistry*
Polymethyl Methacrylate / chemistry*
Surface Properties
Chemical
Reg. No./Substance:
0/Plant Lectins; 0/Polymers; 0/lentil lectin; 11028-71-0/Concanavalin A; 9011-14-7/Polymethyl Methacrylate; EC 3.4.16.5/Carboxypeptidase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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